黏液弯曲杆菌菌株变异的Southern印迹分析。
Southern blot analysis of strain variation in Campylobacter mucosalis.
作者信息
Lin G F, Gebhart C J, Murtaugh M P
机构信息
Department of Veterinary Pathobiology, University of Minnesota, St. Paul 55108.
出版信息
Vet Microbiol. 1991 Feb 1;26(3):279-89. doi: 10.1016/0378-1135(91)90021-7.
A panel of three DNA probes were derived at random from a genomic DNA library of Campylobacter mucosalis strain E8384-4. Each probe hybridized specifically to C. mucosalis DNA from bacteria fixed to nylon membranes. The probes did not hybridize to DNA from other Campylobacter species or to other bacteria even at 100-fold higher amounts. Each probe hybridized to all of 24 isolates of C. mucosalis which had been collected over time from different geographic locations. Southern blot analysis of selected C. mucosalis isolates was carried out to determine if the probes would be useful for differentiating among various isolates. It indicated that restriction fragment length polymorphisms (RFLPs) exist at the loci identified by our probes. These differences were used to characterize seven C. mucosalis isolates recovered from pigs in Minnesota. The results suggest that RFLP analysis may be a useful tool for epidemiological studies of C. mucosalis.
从粘液弯曲杆菌E8384 - 4菌株的基因组DNA文库中随机获得一组三个DNA探针。每个探针都能与固定在尼龙膜上的粘液弯曲杆菌DNA特异性杂交。即使其他弯曲杆菌属物种或其他细菌的DNA量高出100倍，这些探针也不会与之杂交。每个探针都能与24株随时间从不同地理位置收集的粘液弯曲杆菌分离株杂交。对选定的粘液弯曲杆菌分离株进行了Southern印迹分析，以确定这些探针是否有助于区分不同的分离株。结果表明，在我们的探针所识别的位点存在限制性片段长度多态性（RFLP）。这些差异被用于鉴定从明尼苏达州猪身上分离出的7株粘液弯曲杆菌。结果表明，RFLP分析可能是粘液弯曲杆菌流行病学研究的有用工具。
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