Moureau P, Derclaye I, Gregoire D, Janssen M, Cornelis G R
Unité de Microbiologie, Université Catholique de Louvain, Brussels, Belgium.
J Clin Microbiol. 1989 Jul;27(7):1514-7. doi: 10.1128/jcm.27.7.1514-1517.1989.
Total DNA from five Campylobacter species was digested with a mixture of XhoI and BglII restriction endonucleases and analyzed by Southern hybridization by using a probe complementary to the DNA coding for the 16S rRNA. Each of the Campylobacter species, including C. jejuni, C. coli, C. laridis, C. fetus, and C. upsaliensis, displayed a characteristic pattern. Although some bands may be common to different species, the simplicity of the hybridization pattern enabled us to discriminate among the different species of Campylobacter.
用XhoI和BglII限制性内切酶混合物消化五种弯曲杆菌属细菌的总DNA，并使用与编码16S rRNA的DNA互补的探针通过Southern杂交进行分析。每种弯曲杆菌属细菌，包括空肠弯曲杆菌、结肠弯曲杆菌、拉氏弯曲杆菌、胎儿弯曲杆菌和乌普萨拉弯曲杆菌，都呈现出一种特征性模式。尽管不同物种可能有一些共同的条带，但杂交模式的简单性使我们能够区分不同的弯曲杆菌属物种。
