Thébault Sandrine, Deniel Nicolas, Marion Rachel, Charlionet Roland, Tron François, Cosquer David, Leprince Jérôme, Vaudry Hubert, Ducrotté Philippe, Déchelotte Pierre
Groupe ADEN EA3234, Université de Rouen, IFR 23, 22 Boulevard Gambetta, 76183 Rouen, France.
Proteomics. 2006 Jul;6(13):3926-37. doi: 10.1002/pmic.200500714.
Glutamine (Gln) promotes intestinal growth and maintains gut structure and function, especially in situations of injury and during inflammation. Several mechanisms could contribute to Gln protective effects on gut. Proteomics enable us to characterize differentially expressed proteins in tissues in response to modifications of the biological or nutritional environment. Gln effects on the human intestinal epithelial HCT-8 cell line proteome were assessed under basal and proinflammatory conditions. The 2-DE gels were obtained and compared. Proteins were identified by MS and using databases. About 1200 spots were detected in both 2- and 10-mM Gln concentrations. Under basal conditions, 24 proteins were differentially expressed in response to Gln. Half of these proteins were implicated in protein biosynthesis or proteolysis and 20% in membrane trafficking. Under proinflammatory conditions, 27 proteins were up- or down-regulated by Gln 10 mM. From these proteins, 40% were involved in protein biosynthesis or proteolysis, 16% in membrane trafficking, 8% in cell cycle and apoptosis mechanisms and 8% in nucleic acid metabolism. This study provides the first holistic picture of proteome modulation by Gln in a human enterocytic cell line under basal and proinflammatory conditions, and supports further evaluation of nutritional modulation of intestinal proteome in humans.
谷氨酰胺（Gln）可促进肠道生长，并维持肠道结构和功能，尤其是在损伤和炎症期间。有几种机制可能有助于Gln对肠道的保护作用。蛋白质组学使我们能够鉴定出组织中因生物或营养环境改变而差异表达的蛋白质。我们评估了在基础条件和促炎条件下Gln对人肠上皮HCT - 8细胞系蛋白质组的影响。获得并比较了二维凝胶电泳图谱。通过质谱和数据库鉴定蛋白质。在2 mM和10 mM的Gln浓度下均检测到约1200个蛋白点。在基础条件下，有24种蛋白质因Gln而差异表达。其中一半的蛋白质与蛋白质生物合成或蛋白水解有关，20%与膜运输有关。在促炎条件下，10 mM的Gln使27种蛋白质上调或下调。在这些蛋白质中，40%参与蛋白质生物合成或蛋白水解，16%参与膜运输，8%参与细胞周期和凋亡机制，8%参与核酸代谢。本研究首次全面描绘了在基础条件和促炎条件下Gln对人肠细胞系蛋白质组的调节情况，并支持进一步评估人类肠道蛋白质组的营养调节作用。
