de Graauw Marjo, Hensbergen Paul, van de Water Bob
Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Leiden University, The Netherlands.
Electrophoresis. 2006 Jul;27(13):2676-86. doi: 10.1002/elps.200600018.
Reversible protein phosphorylation plays an important role in the regulation of many different processes, such as cell growth, differentiation, migration, metabolism, and apoptosis. Identification of differentially phosphorylated proteins by means of phospho-proteomic analysis provides insight into signal transduction pathways that are activated in response to, for example, growth factor stimulation or toxicant-induced apoptosis. This review summarizes recent advances made in the field of phospho-proteomics and provides examples of how phospho-proteomic techniques can be combined to quantitatively investigate the dynamic changes in protein phosphorylation in time. By linking experimental data to clinical data (e.g., disease progression or response to therapy) new disease markers could be identified, which could then be validated for applications in disease diagnosis and progression or prediction of a response to drugs.
可逆性蛋白质磷酸化在调控许多不同过程中发挥着重要作用，如细胞生长、分化、迁移、代谢及凋亡。通过磷酸化蛋白质组学分析鉴定差异磷酸化蛋白质，有助于深入了解在例如生长因子刺激或毒物诱导凋亡等情况下被激活的信号转导通路。本文综述了磷酸化蛋白质组学领域的最新进展，并举例说明了如何将磷酸化蛋白质组学技术结合起来，以定量研究蛋白质磷酸化的动态变化。通过将实验数据与临床数据（如疾病进展或对治疗的反应）相联系，有可能识别出新的疾病标志物，进而可验证其在疾病诊断、疾病进展或药物反应预测中的应用。
