Paradela Alberto, Albar Juan Pablo
Departamento de Proteömica, Centro Nacional de Biotecnología CSIC, Universidad Autónoma de Madrid, Spain.
Methods Mol Biol. 2006;323:421-9. doi: 10.1385/1-59745-003-0:421.
Large-scale and high-throughput approaches play an essential role in the study of the highly complex biological systems. In plant biology, obtaining the complete genome sequence of the model plant Arabidopsis thaliana has definitively encouraged the incorporation of a completely new set of tools for the identification, characterization, and quantification of its proteome. Proteomics is a new branch of the protein sciences that makes use of these tools and the set of experimental methods specifically designed for them. In this chapter, we describe a complete method for the isolation, fractionation, and identification of a set of proteins obtained from Arabidopsis thaliana cells, stressing methodological aspects that could be of potential interest.
大规模和高通量方法在高度复杂的生物系统研究中发挥着至关重要的作用。在植物生物学领域，模式植物拟南芥完整基因组序列的获得，无疑推动了一系列全新工具的引入，用于其蛋白质组的鉴定、表征和定量分析。蛋白质组学是蛋白质科学的一个新分支，它利用这些工具以及专门为其设计的一系列实验方法。在本章中，我们描述了一种从拟南芥细胞中分离、分级分离和鉴定一组蛋白质的完整方法，重点强调可能具有潜在意义的方法学方面。
