人类脑脊液的蛋白质组学分析

Proteomics analysis of human cerebrospinal fluid.

作者信息

Yuan Xianglin, Desiderio Dominic M

机构信息

Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee Health Science Center, 847 Monroe Avenue, Room 117, Memphis, TN 38163, USA.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 5;815(1-2):179-89. doi: 10.1016/j.jchromb.2004.06.044.

DOI:10.1016/j.jchromb.2004.06.044

PMID:15652808

Abstract

Cerebrospinal fluid (CSF) is secreted from several different central nervous system (CNS) structures, and any changes in the CSF composition will accurately reflect pathological processes. Proteomics offers a comprehensive bird's eye view to analyze CSF proteins at a systems level. This paper reviews the variety of analytical methods that have been used for proteomics analysis of CSF, including sample preparation, two-dimensional liquid and gel electrophoresis, mass spectrometry, bioinformatics, and non-gel methods. The differentially expressed CSF proteins that have been identified by proteomics methods are discussed.

摘要

脑脊液（CSF）由几种不同的中枢神经系统（CNS）结构分泌，脑脊液成分的任何变化都将准确反映病理过程。蛋白质组学提供了一个全面的视角，可在系统水平上分析脑脊液蛋白质。本文综述了用于脑脊液蛋白质组学分析的各种分析方法，包括样品制备、二维液相和凝胶电泳、质谱、生物信息学以及非凝胶方法。还讨论了通过蛋白质组学方法鉴定出的差异表达的脑脊液蛋白质。