通过注射纯化的T7 RNA聚合酶进行卵母细胞表达。
Oocyte expression with injection of purified T7 RNA polymerase.
作者信息
Altafaj Xavier, Joux Nathalie, Ronjat Michel, De Waard Michel
机构信息
Département Réponse et Dynamique Cellulaire, Inserm U607, Grenoble, France.
出版信息
Methods Mol Biol. 2006;322:55-67. doi: 10.1007/978-1-59745-000-3_5.
The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
非洲爪蟾卵母细胞是一种广泛用于蛋白质表达的系统。研究人员可以在两种不同技术之间进行选择：将体外转录的互补RNA（cRNA）注射到细胞质中，或将互补DNA（cDNA）注射到细胞核中。我们报告了第三种表达技术，该技术基于将cDNA和纯化的T7 RNA聚合酶直接联合注射到卵母细胞的细胞质中。
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