Webb D S, Mostowski H S, Gerrard T L
Food and Drug Administration, Center of Biologics Evaluation and Research, Bethesda, MD 20892.
J Immunol. 1991 May 15;146(10):3682-6.
Pretreatment of the human melanoma cell line, A375, and the human colon carcinoma cell line, HT-29, with certain cytokines was found to increase the vulnerability of these cells to monocyte-mediated killing. This activity was found to correlate with increased expression of intercellular adhesion molecule-1 (ICAM-1) on the tumor cells and was blocked by anti-ICAM-1 antibodies. Both IFN-gamma and TNF induced large increases in the ICAM-1 expression on both cell lines and increased the susceptibility of the tumor cells to monocyte-mediated killing. IFN-alpha and IL-1 beta, however, induced only small increases in ICAM-1 expression and enhanced the lysis of the A375 cells but not the HT-29 cells by monocytes. These differences may be the result of a higher basal expression of ICAM-1 found on the A375 cells when compared with the HT-29 cells. These data indicate that regulation of ICAM-1 expression on tumor cells can alter the vulnerability of these cells to lysis by monocytes.
研究发现，用某些细胞因子预处理人黑色素瘤细胞系A375和人结肠癌细胞系HT - 29，会增加这些细胞对单核细胞介导杀伤的易感性。该活性与肿瘤细胞上细胞间黏附分子-1（ICAM - 1）表达的增加相关，并被抗ICAM - 1抗体阻断。干扰素-γ（IFN - γ）和肿瘤坏死因子（TNF）均能使两种细胞系的ICAM - 1表达大幅增加，并提高肿瘤细胞对单核细胞介导杀伤的敏感性。然而，干扰素-α（IFN - α）和白细胞介素-1β（IL - 1β）仅能使ICAM - 1表达小幅增加，且增强了单核细胞对A375细胞的裂解作用，但对HT - 29细胞无此作用。这些差异可能是由于与HT - 29细胞相比，A375细胞上ICAM - 1的基础表达较高。这些数据表明，肿瘤细胞上ICAM - 1表达的调控可改变这些细胞对单核细胞裂解的易感性。
