Gelfi Cecilia, Vigano Agnese, Ripamonti Marilena, Pontoglio Alessandro, Begum Shajna, Pellegrino Maria Antonietta, Grassi Bruno, Bottinelli Roberto, Wait Robin, Cerretelli Paolo
Institute of Molecular Bioimaging and Physiology, CNR, Segrate (MI), Italy.
J Proteome Res. 2006 Jun;5(6):1344-53. doi: 10.1021/pr050414x.
The aim of the present study was to assess age-dependent changes of proteins in the vastus lateralis muscle of physically active elderly and young subjects by a combination of two-dimensional difference gel electrophoresis, SDS-PAGE and ESI-MS/MS. The differences observed in the elderly group included down-regulation of regulatory myosin light chains, particularly the phosphorylated isoforms, a higher proportion of myosin heavy chain isoforms 1 and 2A, and enhanced oxidative and reduced glycolytic capacity.
本研究的目的是通过二维差异凝胶电泳、SDS-PAGE和ESI-MS/MS相结合的方法，评估身体活跃的老年人和年轻人股外侧肌中蛋白质随年龄的变化。在老年组中观察到的差异包括调节性肌球蛋白轻链的下调，特别是磷酸化异构体，肌球蛋白重链异构体1和2A的比例更高，以及氧化能力增强和糖酵解能力降低。
