Kumaraguruparan R, Prathiba D, Nagini S
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608 002, Chidambaram, Tamil Nadu, India.
Res Vet Sci. 2006 Oct;81(2):218-24. doi: 10.1016/j.rvsc.2005.08.002. Epub 2006 Jun 5.
Mammary tumours are the most common neoplasms in humans and canines. Human and canine mammary tumours share several important epidemiological, clinicopathological and biochemical features. Development of mammary tumours involves accumulation of mutant cells caused by excessive proliferation and insufficient apoptosis or dysregulation of cellular differentiation. The present study was therefore designed to investigate the expression of proliferation, differentiation, and apoptosis associated proteins together with expression of estrogen receptors (ER) in both human and canine mammary tumours. Thirty breast cancer patients categorized as pre- and postmenopausal, and 30 mammary gland tumours obtained from bitches were included in this study. The expression of proliferating cell nuclear antigen (PCNA), Bcl-2, p53, cytokeratin and ER in tumour tissues and adjacent tissues were investigated using immunohistochemical staining. While the expression of PCNA, Bcl-2, p53 and ER was significantly increased, expression of cytokeratin was significantly lower in both human as well as canine mammary tumours compared to corresponding adjacent tissues. The magnitude of the changes was however more pronounced in premenopausal patients compared to postmenopausal patients. The changes in proliferation, apoptosis and differentiation associated proteins in human and canine mammary tumours validate use of the canine model to understand the molecular mechanisms of mammary carcinogenesis.
乳腺肿瘤是人类和犬类中最常见的肿瘤。人类和犬类的乳腺肿瘤具有一些重要的流行病学、临床病理和生化特征。乳腺肿瘤的发生涉及因细胞过度增殖、凋亡不足或细胞分化失调导致的突变细胞积累。因此，本研究旨在调查人类和犬类乳腺肿瘤中增殖、分化和凋亡相关蛋白的表达以及雌激素受体（ER）的表达。本研究纳入了30名分为绝经前和绝经后的乳腺癌患者，以及从母犬获得的30个乳腺肿瘤。使用免疫组织化学染色研究肿瘤组织和相邻组织中增殖细胞核抗原（PCNA）、Bcl-2、p53、细胞角蛋白和ER的表达。与相应的相邻组织相比，人类和犬类乳腺肿瘤中PCNA、Bcl-2、p53和ER的表达显著增加，而细胞角蛋白的表达显著降低。然而，与绝经后患者相比，绝经前患者的变化幅度更为明显。人类和犬类乳腺肿瘤中增殖、凋亡和分化相关蛋白的变化证实了使用犬模型来理解乳腺癌发生的分子机制的可行性。
