Cankovic Milena, Gaba Arthur R, Meier Frederick, Kim Wooshin, Zarbo Richard J
Henry Ford Hospital, Detroit, MI 48202, USA.
Gynecol Oncol. 2006 Nov;103(2):614-7. doi: 10.1016/j.ygyno.2006.04.008. Epub 2006 Jun 5.
Gestational and non-gestational choriocarcinomas have distinctly different tissues of origin, parental genotypes, natural histories, and responses to therapy. Our objective was to develop a convenient, fast, and reliable assay that would, using only patient tissue, allow separation of gestational from non-gestational choriocarcinomas.
Benign and malignant tissues, preserved in paraffin blocks and separated by microdissection, were examined using a commercial PCR-based tissue identity assay (ABI AmpFlSTR Profiler Plus Kit and ABI 377 DNA sequencer) to detect genetic profiles of 9 microsatellite markers, along with X and Y chromosome markers. Cases included 6 choriocarcinomas. Controls included eight non-germ cell reproductive tract tumors and two hydatidiform moles.
The microsatellite markers identified the five choriocarcinomas diagnosed on clinical and histological grounds as gestational, to be of genetically non-maternal (androgenic) origin. The neoplasm previously classified as a non-gestational choriocarcinoma was demonstrated to be of maternal origin, as were the non-germ cell reproductive tract tumors. Samples from hydatidiform moles contained either androgenic markers only or a mix of maternal and androgenic markers, as previously seen in complete and partial moles, respectively.
A commercially available microsatellite DNA diagnostic assay is a quick and convenient way to discriminate between gestational and non-gestational choriocarcinoma.
妊娠性和非妊娠性绒毛膜癌在组织起源、亲本基因型、自然病史及对治疗的反应方面存在明显差异。我们的目的是开发一种便捷、快速且可靠的检测方法，仅使用患者组织就能区分妊娠性与非妊娠性绒毛膜癌。
使用基于聚合酶链反应（PCR）的商用组织鉴定检测法（ABI AmpFlSTR Profiler Plus试剂盒和ABI 377 DNA测序仪），对保存在石蜡块中并经显微切割分离的良性和恶性组织进行检测，以检测9个微卫星标记以及X和Y染色体标记的基因图谱。病例包括6例绒毛膜癌。对照包括8例非生殖细胞性生殖道肿瘤和2例葡萄胎。
微卫星标记显示，临床上和组织学上诊断为妊娠性的5例绒毛膜癌，其基因来源为非母源性（雄激素性）。先前分类为非妊娠性绒毛膜癌的肿瘤被证明起源于母方，非生殖细胞性生殖道肿瘤也是如此。葡萄胎样本分别仅含有雄激素性标记或母源性和雄激素性标记的混合，这与之前在完全性和部分性葡萄胎中观察到的情况一致。
一种商用微卫星DNA诊断检测法是区分妊娠性和非妊娠性绒毛膜癌的快速便捷方法。
