嗜热栖热甲烷杆菌Cdc6蛋白与DNA的结合受到微型染色体维持解旋酶的抑制。
DNA binding by the Methanothermobacter thermautotrophicus Cdc6 protein is inhibited by the minichromosome maintenance helicase.
作者信息
Kasiviswanathan Rajesh, Shin Jae-Ho, Kelman Zvi
机构信息
University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, 9600 Gudelsky Drive, Rockville, MD 20850, USA.
出版信息
J Bacteriol. 2006 Jun;188(12):4577-80. doi: 10.1128/JB.00168-06.
Abstract
The Cdc6 proteins from the archaeon Methanothermobacter thermautotrophicus were previously shown to bind double-stranded DNA. It is shown here that the proteins also bind single-stranded DNA. Using minichromosome maintenance (MCM) helicase mutant proteins unable to bind DNA, it was found that the interaction of MCM with Cdc6 inhibits the DNA binding activity of Cdc6.
摘要
先前已表明，嗜热栖热甲烷杆菌古菌中的Cdc6蛋白可结合双链DNA。本文表明，这些蛋白也能结合单链DNA。利用无法结合DNA的微小染色体维持（MCM）解旋酶突变蛋白，研究发现MCM与Cdc6的相互作用会抑制Cdc6的DNA结合活性。
相似文献
1
DNA binding by the Methanothermobacter thermautotrophicus Cdc6 protein is inhibited by the minichromosome maintenance helicase.嗜热栖热甲烷杆菌Cdc6蛋白与DNA的结合受到微型染色体维持解旋酶的抑制。
J Bacteriol. 2006 Jun;188(12):4577-80. doi: 10.1128/JB.00168-06.
2
Interactions between the archaeal Cdc6 and MCM proteins modulate their biochemical properties.古细菌Cdc6蛋白与MCM蛋白之间的相互作用调节了它们的生化特性。
Nucleic Acids Res. 2005 Sep 8;33(15):4940-50. doi: 10.1093/nar/gki807. Print 2005.
3
The Methanothermobacter thermautotrophicus Cdc6-2 protein, the putative helicase loader, dissociates the minichromosome maintenance helicase.嗜热自养甲烷杆菌的Cdc6-2蛋白，即假定的解旋酶装载蛋白，可使微小染色体维持解旋酶解离。
J Bacteriol. 2008 Jun;190(11):4091-4. doi: 10.1128/JB.00233-08. Epub 2008 Apr 4.
4
Archaeal minichromosome maintenance (MCM) helicase can unwind DNA bound by archaeal histones and transcription factors.古菌微小染色体维持（MCM）解旋酶能够解开与古菌组蛋白和转录因子结合的DNA。
J Biol Chem. 2007 Feb 16;282(7):4908-4915. doi: 10.1074/jbc.M606847200. Epub 2006 Dec 9.
5
Stimulation of MCM helicase activity by a Cdc6 protein in the archaeon Thermoplasma acidophilum.嗜热栖热菌中Cdc6蛋白对MCM解旋酶活性的刺激作用。
Nucleic Acids Res. 2006;34(21):6337-44. doi: 10.1093/nar/gkl864. Epub 2006 Nov 15.
6
Coupling of DNA binding and helicase activity is mediated by a conserved loop in the MCM protein.DNA结合与解旋酶活性的偶联由MCM蛋白中的一个保守环介导。
Nucleic Acids Res. 2008 Mar;36(4):1309-20. doi: 10.1093/nar/gkm1160. Epub 2008 Jan 9.
7
Regulation of minichromosome maintenance helicase activity by Cdc6.Cdc6对微小染色体维持解旋酶活性的调控。
J Biol Chem. 2003 Sep 26;278(39):38059-67. doi: 10.1074/jbc.M305477200. Epub 2003 Jul 1.
8
High-temperature single-molecule kinetic analysis of thermophilic archaeal MCM helicases.嗜热古菌MCM解旋酶的高温单分子动力学分析
Nucleic Acids Res. 2016 Oct 14;44(18):8764-8771. doi: 10.1093/nar/gkw612. Epub 2016 Jul 5.
9
The Methanothermobacter thermautotrophicus MCM helicase is active as a hexameric ring.嗜热自养甲烷杆菌MCM解旋酶以六聚体环的形式具有活性。
J Biol Chem. 2009 Jan 2;284(1):540-546. doi: 10.1074/jbc.M806803200. Epub 2008 Nov 10.
10
ATP hydrolysis and DNA binding confer thermostability on the MCM helicase.ATP 水解和 DNA 结合赋予 MCM 解旋酶热稳定性。
Biochemistry. 2009 Mar 24;48(11):2330-9. doi: 10.1021/bi801921j.
引用本文的文献
1
Atomic Force Microscopy Investigation of the Interactions between the MCM Helicase and DNA.MCM解旋酶与DNA相互作用的原子力显微镜研究
Materials (Basel). 2021 Feb 2;14(3):687. doi: 10.3390/ma14030687.
2
Characterization of the replication initiator Orc1/Cdc6 from the Archaeon Picrophilus torridus.热球菌古菌 Orc1/Cdc6 复制起始因子的特性分析。
J Bacteriol. 2014 Jan;196(2):276-86. doi: 10.1128/JB.01020-13. Epub 2013 Nov 1.
3
Mutational analysis of conserved aspartic acid residues in the Methanothermobacter thermautotrophicus MCM helicase.产甲烷热原体 MCM 解旋酶保守天冬氨酸残基的突变分析。
Extremophiles. 2011 Mar;15(2):245-52. doi: 10.1007/s00792-010-0352-1. Epub 2011 Jan 28.
4
Biochemical characterization of the minichromosome maintenance protein from the archaeon Thermoplasma acidophilum.嗜酸嗜热栖热袍菌微小染色体维持蛋白的生化特性
Extremophiles. 2009 Jan;13(1):81-8. doi: 10.1007/s00792-008-0198-y. Epub 2008 Nov 11.
5
Thermoplasma acidophilum Cdc6 protein stimulates MCM helicase activity by regulating its ATPase activity.嗜热栖热放线菌Cdc6蛋白通过调节其ATP酶活性来刺激MCM解旋酶活性。
Nucleic Acids Res. 2008 Oct;36(17):5602-9. doi: 10.1093/nar/gkn548. Epub 2008 Aug 30.
6
The Methanothermobacter thermautotrophicus Cdc6-2 protein, the putative helicase loader, dissociates the minichromosome maintenance helicase.嗜热自养甲烷杆菌的Cdc6-2蛋白，即假定的解旋酶装载蛋白，可使微小染色体维持解旋酶解离。
J Bacteriol. 2008 Jun;190(11):4091-4. doi: 10.1128/JB.00233-08. Epub 2008 Apr 4.
7
Coupling of DNA binding and helicase activity is mediated by a conserved loop in the MCM protein.DNA结合与解旋酶活性的偶联由MCM蛋白中的一个保守环介导。
Nucleic Acids Res. 2008 Mar;36(4):1309-20. doi: 10.1093/nar/gkm1160. Epub 2008 Jan 9.
本文引用的文献
1
Archaeal DNA replication and repair.古菌DNA复制与修复。
Curr Opin Microbiol. 2005 Dec;8(6):669-76. doi: 10.1016/j.mib.2005.10.001. Epub 2005 Oct 20.
2
Interactions between the archaeal Cdc6 and MCM proteins modulate their biochemical properties.古细菌Cdc6蛋白与MCM蛋白之间的相互作用调节了它们的生化特性。
Nucleic Acids Res. 2005 Sep 8;33(15):4940-50. doi: 10.1093/nar/gki807. Print 2005.
3
Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernix.来自嗜热栖热菌的Cdc6/ORC中核苷酸结合诱导的构象变化。
J Mol Biol. 2004 Oct 22;343(3):547-57. doi: 10.1016/j.jmb.2004.08.044.
4
Biochemical characterization of Cdc6/Orc1 binding to the replication origin of the euryarchaeon Methanothermobacter thermoautotrophicus.嗜热自养甲烷杆菌Cdc6/Orc1与复制起点结合的生化特性研究
Nucleic Acids Res. 2004 Sep 9;32(16):4821-32. doi: 10.1093/nar/gkh819. Print 2004.
5
Three replication origins in Sulfolobus species: synchronous initiation of chromosome replication and asynchronous termination.硫化叶菌属物种中的三个复制起点：染色体复制的同步起始和异步终止
Proc Natl Acad Sci U S A. 2004 May 4;101(18):7046-51. doi: 10.1073/pnas.0400656101. Epub 2004 Apr 23.
6
Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus.嗜热栖热菌单染色体中两个复制起点的鉴定。
Cell. 2004 Jan 9;116(1):25-38. doi: 10.1016/s0092-8674(03)01034-1.
7
Replicative helicase loaders: ring breakers and ring makers.复制性解旋酶装载蛋白：环破坏者与环制造者
Curr Biol. 2003 Aug 5;13(15):R594-6. doi: 10.1016/s0960-9822(03)00523-2.
8
Regulation of minichromosome maintenance helicase activity by Cdc6.Cdc6对微小染色体维持解旋酶活性的调控。
J Biol Chem. 2003 Sep 26;278(39):38059-67. doi: 10.1074/jbc.M305477200. Epub 2003 Jul 1.
9
Archaea: an archetype for replication initiation studies?古菌：复制起始研究的原型？
Mol Microbiol. 2003 May;48(3):605-15. doi: 10.1046/j.1365-2958.2003.03369.x.
10
Common domains in the initiators of DNA replication in Bacteria, Archaea and Eukarya: combined structural, functional and phylogenetic perspectives.细菌、古菌和真核生物中DNA复制起始因子的常见结构域：结构、功能和系统发育的综合视角
FEMS Microbiol Rev. 2003 Jan;26(5):533-54. doi: 10.1111/j.1574-6976.2003.tb00629.x.
Zotero 插件