细胞色素 b(5)在大鼠肝微粒体中的特性与生物合成。
Characterization and biosynthesis of cytochrome b(5) in rat liver microsomes.
机构信息
Department of Biological Chemistry, Marischal College, University of Aberdeen.
出版信息
Biochem J. 1968 May;107(6):839-49. doi: 10.1042/bj1070839.
Abstract
- Cytochrome b(5) is released from rat liver microsomes by both proteolytic enzymes and by treatments that disrupt phospholipids. Cytochrome P-420 is only released to a marked extent by treatments that disrupt phospholipids. 2. Cytochrome b(5) was isolated in a pure state from both the rough and smooth fractions of rat liver microsomes after treatment with trypsin, and was shown to contain two cytochrome components with identical spectral properties. 3. Amino acid analyses of the two components are presented, together with peptide ;fingerprint' patterns of tryptic digests of the two components. 4. Studies based on the direct isolation of cytochrome b(5) after administration of a single dose of radioactive amino acid to rats demonstrate that the cytochrome is synthesized initially in the rough fraction of microsomes and only subsequently appears in the smooth fraction. 5. Isolated rat liver microsomes are capable of incorporating radioactive amino acids into cytochrome b(5) under standard conditions. 6. Under these conditions the amino acid is incorporated into peptide linkage in the cytochrome.
摘要
细胞色素 b(5) 既可被蛋白水解酶，也可被破坏磷脂的处理从大鼠肝微粒体中释放出来。细胞色素 P-420 仅在破坏磷脂的处理下才大量释放。
用胰蛋白酶处理大鼠肝微粒体的粗面和光滑部分后，可从这两部分纯分离出细胞色素 b(5)，并证明它含有两个具有相同光谱特性的细胞色素成分。
提出了这两个成分的氨基酸分析结果，以及这两个成分胰蛋白酶消化物的肽“指纹”模式。
基于单次给予放射性氨基酸后直接分离细胞色素 b(5) 的研究表明，该细胞色素最初在微粒体的粗面部分合成，随后才出现在光滑部分。
分离的大鼠肝微粒体在标准条件下能够将放射性氨基酸掺入细胞色素 b(5) 中。
在这些条件下，氨基酸被掺入细胞色素的肽键中。
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