大鼠肝脏微粒体和分离的亚细胞膜的分析研究。V. 细胞色素b5的免疫电镜定位：方法及其在各种亚细胞组分中的应用
Analytical study of microsomes and isolated subcellular membranes from rat liver. V. Immunological localization of cytochrome b5 by electron microscopy: methodology and application to various subcellular fractions.
作者信息
Fowler S, Remacle J, Trouet A, Beaufay H, Berthet J, Wibo M, Hauser P
出版信息
J Cell Biol. 1976 Nov;71(2):535-50. doi: 10.1083/jcb.71.2.535.
Abstract
The localization of cytochrome b5 on the membranes of various subcellular organelles of rat liver was studied by a cytoimmunological procedure using anti-cytochrome b5/anti-ferritin hybrid antibodies and ferritin as label. For this study, highly purified and biochemically characterized membrane preparations were employed. Outer mitochondrial membranes were found to be heavily labeled by the hybrid antibodies whereas Golgi and plasma membranes were not marked by the reagent. Peroxisome membranes were moderately labeled by the hybrid antibodies, suggesting that they may contain some cytochrome b5. The preparation and purification of hybrid antibodies without peptic digestion is described and an analysis made of the composition of the final reagent product.
摘要
采用抗细胞色素b5/抗铁蛋白杂交抗体并以铁蛋白作为标记，通过细胞免疫程序研究了大鼠肝脏各种亚细胞细胞器膜上细胞色素b5的定位。在本研究中，使用了高度纯化且经过生化特性鉴定的膜制剂。发现线粒体外膜被杂交抗体大量标记，而高尔基体膜和质膜未被该试剂标记。过氧化物酶体膜被杂交抗体适度标记，表明它们可能含有一些细胞色素b5。描述了未经胃蛋白酶消化的杂交抗体的制备和纯化，并对最终试剂产物的组成进行了分析。
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Analytical study of microsomes and isolated subcellular membranes from rat liver. V. Immunological localization of cytochrome b5 by electron microscopy: methodology and application to various subcellular fractions.大鼠肝脏微粒体和分离的亚细胞膜的分析研究。V. 细胞色素b5的免疫电镜定位：方法及其在各种亚细胞组分中的应用
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引用本文的文献
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3
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本文引用的文献
1
Characterization and biosynthesis of cytochrome b(5) in rat liver microsomes.细胞色素 b(5)在大鼠肝微粒体中的特性与生物合成。
Biochem J. 1968 May;107(6):839-49. doi: 10.1042/bj1070839.
2
A Hemochromogen Component of Liver Microsomes.肝微粒体的一种血色原成分。
Proc Natl Acad Sci U S A. 1952 Jan;38(1):19-25. doi: 10.1073/pnas.38.1.19.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
EVIDENCE FOR LINKAGE OF UNIVALENT FRAGMENTS OR HALF-MOLECULES OF RABBIT GAMMA-GLOBULIN BY THE SAME DISULFIDE BOND.兔γ球蛋白单价片段或半分子通过同一二硫键连接的证据。
Biochemistry. 1964 Sep;3:1338-42. doi: 10.1021/bi00897a025.
5
HYBRIDIZATION OF HALF MOLECULES OF RABBIT GAMMA GLOBULIN.兔γ球蛋白半分子的杂交
Science. 1964 Jan 24;143(3604):376-9. doi: 10.1126/science.143.3604.376.
6
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
7
Preparation of an electron-dense antibody conjugate.电子致密抗体偶联物的制备。
Nature. 1959 May 30;183(4674):1523-4. doi: 10.1038/1831523a0.
8
A quantitative immunochemical measure of the primary interaction between I BSA and antibody.一种对牛血清白蛋白（BSA）与抗体之间主要相互作用的定量免疫化学测定方法。
J Infect Dis. 1958 Nov-Dec;103(3):239-62. doi: 10.1093/infdis/103.3.239.
9
Kinetics of cytochrome b5 in rat liver microsomes.大鼠肝脏微粒体中细胞色素b5的动力学
J Biol Chem. 1954 Aug;209(2):945-51.
10
Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles.亚细胞组分的电子显微镜检查。I. 从颗粒悬浮液中制备代表性样品。
J Cell Biol. 1967 Jan;32(1):181-91. doi: 10.1083/jcb.32.1.181.
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