Fowler S, Remacle J, Trouet A, Beaufay H, Berthet J, Wibo M, Hauser P
J Cell Biol. 1976 Nov;71(2):535-50. doi: 10.1083/jcb.71.2.535.
The localization of cytochrome b5 on the membranes of various subcellular organelles of rat liver was studied by a cytoimmunological procedure using anti-cytochrome b5/anti-ferritin hybrid antibodies and ferritin as label. For this study, highly purified and biochemically characterized membrane preparations were employed. Outer mitochondrial membranes were found to be heavily labeled by the hybrid antibodies whereas Golgi and plasma membranes were not marked by the reagent. Peroxisome membranes were moderately labeled by the hybrid antibodies, suggesting that they may contain some cytochrome b5. The preparation and purification of hybrid antibodies without peptic digestion is described and an analysis made of the composition of the final reagent product.
采用抗细胞色素b5/抗铁蛋白杂交抗体并以铁蛋白作为标记，通过细胞免疫程序研究了大鼠肝脏各种亚细胞细胞器膜上细胞色素b5的定位。在本研究中，使用了高度纯化且经过生化特性鉴定的膜制剂。发现线粒体外膜被杂交抗体大量标记，而高尔基体膜和质膜未被该试剂标记。过氧化物酶体膜被杂交抗体适度标记，表明它们可能含有一些细胞色素b5。描述了未经胃蛋白酶消化的杂交抗体的制备和纯化，并对最终试剂产物的组成进行了分析。
