大肠杆菌对葡萄糖酸盐的利用。葡萄糖酸激酶和 6-磷酸葡萄糖酸脱水酶活性的诱导。

Utilization of gluconate by Escherichia coli. Induction of gluconate kinase and 6-phosphogluconate dehydratase activities.

Department of Biochemistry, School of Biological Sciences, University of Leicester, Leicester LE1 7RH, U.K.

Biochem J. 1973 Jun;134(2):489-98. doi: 10.1042/bj1340489.

Abstract

A mutant of Escherichia coli, devoid of phosphopyruvate synthetase, glucosephosphate isomerase and 6-phosphogluconate dehydrogenase activities, grew readily on gluconate and inducibly formed an uptake system for gluconate, gluconate kinase and 6-phosphogluconate dehydratase while doing so. 2. This mutant also grew on glucose 6-phosphate and inducibly formed 6-phosphogluconate dehydratase; however, the formation of the gluconate uptake system and gluconate kinase was not induced under these conditions. 3. The use of the Entner-Doudoroff pathway for the dissimilation of 6-phosphogluconate, derived from either gluconate or glucose 6-phosphate, by this mutant was also demonstrated by the accumulation of 2-keto-3-deoxy-6-phosphogluconate (3-deoxy-6-phospho-l-glycero-2-hexulosonate) from both these substrates in a similar mutant that also lacked phospho-2-keto-3-deoxygluconate aldolase activity. 4. Glucose 6-phosphate inhibits the continued utilization of fructose by cultures of the mutants growing on fructose, as it does in wild-type E. coli. 5. The mutants do not use glucose for growth. This is shown to be due to insufficiency of phosphopyruvate, which is required for glucose uptake.

摘要

一种缺乏磷酸烯醇丙酮酸合成酶、磷酸葡萄糖异构酶和 6-磷酸葡萄糖酸脱氢酶活性的大肠杆菌突变体，能在葡萄糖酸盐上迅速生长，并在诱导下形成葡萄糖酸盐摄取系统、葡萄糖酸激酶和 6-磷酸葡萄糖酸脱水酶。
该突变体也能在葡萄糖 6-磷酸上生长，并在诱导下形成 6-磷酸葡萄糖酸脱水酶；然而，在这些条件下，葡萄糖酸盐摄取系统和葡萄糖酸激酶的形成没有被诱导。
通过在另一种也缺乏磷酸-2-酮-3-脱氧葡萄糖酸醛缩酶活性的类似突变体中积累 2-酮-3-脱氧-6-磷酸葡萄糖酸（3-脱氧-6-磷酸-l-甘油-2-己酮糖），证明了该突变体可以利用来自葡萄糖酸盐或葡萄糖 6-磷酸的 6-磷酸葡萄糖通过 Entner-Doudoroff 途径进行异化。
葡萄糖 6-磷酸抑制了在果糖上生长的突变体培养物继续利用果糖，就像在野生型大肠杆菌中一样。
突变体不能利用葡萄糖进行生长。这是由于缺乏磷酸烯醇丙酮酸，这是葡萄糖摄取所必需的。