大鼠大脑皮层腺苷脱氨酶活性及其亚细胞分布。
Rat cerebral-cortex adenosine deaminase activity and its subcellular distribution.
作者信息
Pull I, McIlwain H
出版信息
Biochem J. 1974 Oct;144(1):37-41. doi: 10.1042/bj1440037.
Abstract
A radioisotopic assay for adenosine deaminase (EC 3.5.4.4) is described together with its application in investigating the activity of the enzyme in rat cerebral cortex. Activity of the adenosine deaminase was determined to be 115nmol/min per g of tissue, measured in isoosmotic sucrose dispersions of the neocortex, and to be 170nmol/min per g of tissue after treatment with Triton X-100. The enzyme was concluded to be largely cytoplasmic, with a K(m) of 54-57mum for adenosine. Action of the deaminase, and other aspects of the metabolism of adenosine in intact neocortical tissue, were quantitatively appraised on the basis of the newly determined characteristics.
摘要
本文描述了一种用于腺苷脱氨酶（EC 3.5.4.4）的放射性同位素测定法及其在研究大鼠大脑皮层中该酶活性的应用。在新皮层的等渗蔗糖分散液中测得，腺苷脱氨酶的活性为每克组织115nmol/分钟，在用 Triton X - 100处理后为每克组织170nmol/分钟。该酶被认为主要存在于细胞质中，对腺苷的K(m)为54 - 57μm。基于新确定的特性，对完整新皮层组织中脱氨酶的作用以及腺苷代谢的其他方面进行了定量评估。
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