一种测定尿中孕酮6-氧代代谢物的方法。
A method for the estimation of 6-oxygenated metabolites of progesterone in urine.
作者信息
James F, Fotherby K
机构信息
Postgraduate Medical School, Ducane Road, London, W. 12.
出版信息
Biochem J. 1965 May;95(2):459-65. doi: 10.1042/bj0950459.
Abstract
- A method is described for the estimation of the 6-oxygenated metabolites of progesterone in urine. After hydrolysis the extract of urine is chromatographed on alumina to obtain a fraction containing mainly the 6-oxygenated metabolites. This fraction is oxidized to convert the metabolites into pregnane-3,6,20-triones, which are estimated as the dinitrophenylhydrazones. 2. The reliability criteria of the method are presented. Normal subjects excrete 0.1-0.6mg./day, and at the end of pregnancy values of 3.3-11.6mg./day are obtained.
摘要
- 本文描述了一种估算尿中孕酮6-氧化代谢物的方法。尿液提取物经水解后在氧化铝上进行色谱分离，以获得主要含有6-氧化代谢物的馏分。该馏分被氧化，将代谢物转化为孕烷-3,6,20-三酮，以二硝基苯腙形式进行估算。2. 给出了该方法的可靠性标准。正常受试者每日排泄量为0.1 - 0.6毫克，妊娠末期的值为每日3.3 - 11.6毫克。
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本文引用的文献
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