一种估算尿睾酮的方法。
A method for the estimation of urinary testosterone.
作者信息
Ismail A A, Harkness R A
出版信息
Biochem J. 1966 Jun;99(3):717-25. doi: 10.1042/bj0990717.
Abstract
- A method has been developed for the estimation of testosterone in human urine by using acid hydrolysis followed by a quantitative form of a modified Girard reaction that separates a ;conjugated-ketone' fraction from a urine extract; this is followed by column chromatography on alumina and paper chromatography. 2. Comparison of methods of estimation of testosterone in the final fraction shows that estimation by gas-liquid chromatography is more reproducible than by colorimetric methods applied to the same eluates from the paper chromatogram. 3. The mean recovery of testosterone by gas-liquid chromatography is 79.5%, and this method appears to be specific for testosterone. 4. The procedure is relatively rapid. Six determinations can be performed by one worker in 2 days. 5. Results of determinations on human urine are briefly presented. In general, they are similar to earlier estimates, but the maximal values are lower.
摘要
- 已开发出一种通过酸水解，随后采用改良吉拉德反应的定量形式从尿液提取物中分离出“共轭酮”部分，再进行氧化铝柱色谱和纸色谱来估算人尿中睾酮的方法。2. 对最终馏分中睾酮估算方法的比较表明，气液色谱法估算比应用于纸色谱相同洗脱液的比色法更具重现性。3. 气液色谱法测定睾酮的平均回收率为79.5%，该方法似乎对睾酮具有特异性。4. 该方法相对快速。一名工作人员在2天内可进行6次测定。5. 简要介绍了人尿测定结果。总体而言，它们与早期估算结果相似，但最大值较低。
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