Jiang Jia-fu, Wu Xiao-ming, Wang Ri-ming, Zuo Shu-qing, Xu Wei-cai, Guo Tian-yu, Chen Li-quan, Cao Wu-chun
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2006 Feb;27(2):145-9.
In order to find out the factors related to hemorrhagic fever with renal syndrome (HFRS) infection, and to evaluate the probability of ecdemic hantaviruses (HV) infection in rodents in Beijing areas.
Rodents were collected in a large-scale railway station and a produce market with 'trap nights' method from April to May, 2004. The IgG reacting sera to HV antigen were detected using ELISA. The partial M and S segment of HV from captured rodent lung samples were amplified with RT-PCR. The PCR products were purified and sequenced. BLAST program was then used to perform on nucleotide pairwise alignment with all available sequence in GenBank. The alignment of the multiply nucleotide and the deduced amino acid sequences, together with phylogenetic analysis were completed with DNASTAR software.
The average population density was 3.49% (24/690). The overall seroprevalence of HV infection was 8.3% (2/24). RT-PCR positive rates were 8.3% (2/24). The nucleotide sequences of 356 bp region (1958 - 2313) of M segment obtained from 2 samples were all identified to Seoul virus (SEOV), with 7.6% heterogeneity. The dc501 strain from railway station was closely related to SD227 and Hebei4 from Shandong and Hebei provinces respectively. BjFT01 strain from the farm product market had more special nucleotide transitional mutations than other known SEOV from Beijing in GenBank. This strain, together with known HN71 from Hainan province, K24-E7 from Zhejiang province, L99 from Jiangxi province and R22 from Henan province, represented a monophylogentic linkage.
The higher HV prevalence of rodents in transportation center was the potential and important risk for HFRS epidemic in Beijing. The increasing prevalence of M. musculus should call for attention. It was possible that SEOV in Beijing was imported by infected rodents through vehicles from other provinces.
了解肾综合征出血热（HFRS）感染的相关因素，评估北京地区啮齿动物中流行性汉坦病毒（HV）感染的可能性。
2004年4月至5月，采用“夹夜法”在某大型火车站和某农产品市场采集啮齿动物。用酶联免疫吸附测定法（ELISA）检测血清中对HV抗原的IgG反应性。用逆转录-聚合酶链反应（RT-PCR）扩增捕获的啮齿动物肺组织样本中HV的部分M和S片段。对PCR产物进行纯化和测序。然后使用BLAST程序对GenBank中所有可用序列进行核苷酸两两比对。用DNASTAR软件完成多个核苷酸和推导氨基酸序列的比对以及系统发育分析。
平均鼠密度为3.49%（24/690）。HV感染的总体血清阳性率为8.3%（2/24）。RT-PCR阳性率为8.3%（2/24）。从2份样本中获得的M片段356bp区域（1958 - 2313）的核苷酸序列均鉴定为汉城病毒（SEOV），异质性为7.6%。火车站的dc501株分别与来自山东和河北的SD227和河北4株密切相关。农产品市场的BjFT01株比GenBank中北京已知的其他SEOV有更多特殊的核苷酸转换突变。该毒株与来自海南省的已知HN71、浙江省的K24-E7、江西省的L99和河南省的R22构成一个单系分支。
交通枢纽地区啮齿动物中HV流行率较高是北京HFRS流行的潜在重要风险。小家鼠流行率的上升应引起关注。北京的SEOV有可能是被感染的啮齿动物通过车辆从其他省份传入的。
