Suppr超能文献

低pH值下生物膜中的硝化作用:原位微环境和耐酸性的作用

Nitrification in a biofilm at low pH values: role of in situ microenvironments and acid tolerance.

作者信息

Gieseke Armin, Tarre Sheldon, Green Michal, de Beer Dirk

机构信息

Microsensor Group, Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D-28359 Bremen, Germany.

出版信息

Appl Environ Microbiol. 2006 Jun;72(6):4283-92. doi: 10.1128/AEM.00241-06.

Abstract

The sensitivity of nitrifying bacteria to acidic conditions is a well-known phenomenon and generally attributed to the lack and/or toxicity of substrates (NH3 and HNO2) with decreasing pHs. In contrast, we observed strong nitrification at a pH around 4 in biofilms grown on chalk particles and investigated the following hypotheses: the presence of less acidic microenvironments and/or the existence of acid-tolerant nitrifiers. Microelectrode measurements (in situ and under various experimental conditions) showed no evidence of a neutral microenvironment, either within the highly active biofilm colonizing the chalk surface or within a control biofilm grown on a nonbuffering (i.e., sintered glass) surface under acidic pH. A 16S rRNA approach (clone libraries and fluorescence in situ hybridizations) did not reveal uncommon nitrifying (potentially acid-tolerant) strains. Instead, we found a strongly acidic microenvironment, evidence for a clear adaptation to the low pH in situ, and the presence of nitrifying populations related to subgroups with low Km s for ammonia (Nitrosopira spp., Nitrosomonas oligotropha, and Nitrospira spp.). Acid-consuming (chalk dissolution) and acid-producing (ammonia oxidation) processes are equilibrated on a low-pH steady state that is controlled by mass transfer limitation through the biofilm. Strong affinity to ammonia and possibly the expression of additional functions, e.g., ammonium transporters, are adaptations that allow nitrifiers to cope with acidic conditions in biofilms and other habitats.

摘要

硝化细菌对酸性条件的敏感性是一个众所周知的现象,通常归因于随着pH值降低底物(NH3和HNO2)的缺乏和/或毒性。相比之下,我们观察到在白垩颗粒上生长的生物膜在pH约为4时具有强烈的硝化作用,并研究了以下假设:存在酸性较弱的微环境和/或存在耐酸硝化菌。微电极测量(原位和在各种实验条件下)表明,在定殖于白垩表面的高活性生物膜内或在酸性pH下在非缓冲(即烧结玻璃)表面上生长的对照生物膜内,均没有中性微环境的迹象。16S rRNA方法(克隆文库和荧光原位杂交)未揭示不常见的硝化(潜在耐酸)菌株。相反,我们发现了一个强酸性微环境,有证据表明其在原位对低pH有明显适应性,并且存在与对氨具有低Km值的亚群相关的硝化菌群(亚硝化螺菌属、寡营养亚硝化单胞菌和硝化螺菌属)。耗酸(白垩溶解)和产酸(氨氧化)过程在一个低pH稳态下达到平衡,该稳态由通过生物膜的传质限制控制。对氨的强亲和力以及可能的其他功能(例如铵转运蛋白)的表达是使硝化菌能够应对生物膜和其他生境中酸性条件的适应性特征。

相似文献

1
Nitrification in a biofilm at low pH values: role of in situ microenvironments and acid tolerance.
Appl Environ Microbiol. 2006 Jun;72(6):4283-92. doi: 10.1128/AEM.00241-06.
3
Community structure and activity dynamics of nitrifying bacteria in a phosphate-removing biofilm.
Appl Environ Microbiol. 2001 Mar;67(3):1351-62. doi: 10.1128/AEM.67.3.1351-1362.2001.
4
High-rate nitrification at low pH in suspended- and attached-biomass reactors.
Appl Environ Microbiol. 2004 Nov;70(11):6481-7. doi: 10.1128/AEM.70.11.6481-6487.2004.
5
Identification of bacteria responsible for ammonia oxidation in freshwater aquaria.
Appl Environ Microbiol. 2001 Dec;67(12):5791-800. doi: 10.1128/AEM.67.12.5791-5800.2001.
7
Vertical distribution of nitrifying populations in bacterial biofilms from a full-scale nitrifying trickling filter.
Environ Microbiol. 2006 Nov;8(11):2036-49. doi: 10.1111/j.1462-2920.2006.01085.x.
8
Microenvironments and distribution of nitrifying bacteria in a membrane-bound biofilm.
Environ Microbiol. 2000 Dec;2(6):680-6. doi: 10.1046/j.1462-2920.2000.00150.x.

引用本文的文献

1
Comammox act as key bacteria in weakly acidic soil via potential cobalamin sharing.
Imeta. 2025 Feb 4;4(1):e271. doi: 10.1002/imt2.271. eCollection 2025 Feb.
3
Comammox Nitrospira was the dominant ammonia oxidizer in an acidic biofilm reactor at pH 5.5 and pH 5.
Appl Microbiol Biotechnol. 2024 Oct 24;108(1):494. doi: 10.1007/s00253-024-13306-z.
4
Comammox and Ammonia-Oxidizing Archaea Are Dominant Ammonia Oxidizers in Sediments of an Acid Mine Lake Containing High Ammonium Concentrations.
Appl Environ Microbiol. 2023 Mar 29;89(3):e0004723. doi: 10.1128/aem.00047-23. Epub 2023 Mar 13.
8
Shifts in methanogenic archaea communities and methane dynamics along a subtropical estuarine land use gradient.
PLoS One. 2020 Nov 24;15(11):e0242339. doi: 10.1371/journal.pone.0242339. eCollection 2020.
9
Low Temperature and Neutral pH Define " Nitrotoga sp." as a Competitive Nitrite Oxidizer in Coculture with Nitrospira defluvii.
Appl Environ Microbiol. 2019 Apr 18;85(9). doi: 10.1128/AEM.02569-18. Print 2019 May 1.
10
Growth of Nitrosococcus-Related Ammonia Oxidizing Bacteria Coincides with Extremely Low pH Values in Wastewater with High Ammonia Content.
Environ Sci Technol. 2017 Jun 20;51(12):6857-6866. doi: 10.1021/acs.est.7b00392. Epub 2017 Jun 5.

本文引用的文献

2
A nitrite microsensor for profiling environmental biofilms.
Appl Environ Microbiol. 1997 Mar;63(3):973-7. doi: 10.1128/aem.63.3.973-977.1997.
4
In situ substrate conversion and assimilation by nitrifying bacteria in a model biofilm.
Environ Microbiol. 2005 Sep;7(9):1392-404. doi: 10.1111/j.1462-2920.2005.00826.x.
6
High-rate nitrification at low pH in suspended- and attached-biomass reactors.
Appl Environ Microbiol. 2004 Nov;70(11):6481-7. doi: 10.1128/AEM.70.11.6481-6487.2004.
8
ARB: a software environment for sequence data.
Nucleic Acids Res. 2004 Feb 25;32(4):1363-71. doi: 10.1093/nar/gkh293. Print 2004.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验