单核细胞增生李斯特菌的固定与检测
Immobilization and detection of Listeria monocytogenes.
作者信息
Schindler B D, Shelef L A
机构信息
Department of Nutrition and Food Science, Wayne State University, Detroit, MI 48202, USA.
出版信息
Appl Environ Microbiol. 2006 Jun;72(6):4426-8. doi: 10.1128/AEM.02809-05.
Abstract
A procedure was developed for immobilization of Listeria monocytogenes cells on metal hydroxides coupled with detection and enumeration using an automated optical system. The results of the immobilization procedure (<1 h) and detection during overnight incubation agreed with calculated plate counts, and this technique is simple and rapid and provides samples that are ready for confirmation of the presence of the pathogen by rapid methods.
摘要
开发了一种将单核细胞增生李斯特氏菌细胞固定在金属氢氧化物上的方法，并结合使用自动光学系统进行检测和计数。固定化过程（<1小时）的结果以及过夜培养期间的检测结果与计算得出的平板计数结果一致，该技术简单快速，并且能提供可供通过快速方法确认病原体存在的样本。
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