多重聚合酶链反应用于同时检测李斯特菌属细菌、单核细胞增生李斯特菌以及单核细胞增生李斯特菌的主要血清型和流行克隆。
Multiplex PCR for simultaneous detection of bacteria of the genus Listeria, Listeria monocytogenes, and major serotypes and epidemic clones of L. monocytogenes.
作者信息
Chen Yi, Knabel Stephen J
机构信息
The Pennsylvania State University, Food Science Department, 436 Food Science Building, University Park, PA 16802, USA.
出版信息
Appl Environ Microbiol. 2007 Oct;73(19):6299-304. doi: 10.1128/AEM.00961-07. Epub 2007 Aug 10.
Abstract
A multiplex PCR assay which combines detection of bacteria of the genus Listeria, Listeria monocytogenes serotypes 1/2a and 4b, and epidemic clones I, II, and III of L. monocytogenes was developed. The assay provides a rapid, reliable, and inexpensive method for screening and subgrouping this important food-borne pathogen.
摘要
开发了一种多重聚合酶链反应（PCR）检测方法，该方法可同时检测李斯特菌属细菌、单核细胞增生李斯特菌1/2a和4b血清型以及单核细胞增生李斯特菌的流行克隆I、II和III。该检测方法为筛选和细分这种重要的食源性病原体提供了一种快速、可靠且廉价的方法。
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