Krause P H, Schmitz R, Lindemann M, Georgii A
Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1975;83(3):177-86. doi: 10.1007/BF00304085.
Successful xenotransplantation of 5 different established tumor cell-lines was performed in the congenital thymusless mouse mutant "nude". The tumors were one human carcinoma of the cervix (HeLa), one Burkitt's lymphoma as well as two Polyoma virus-induced tumors from rats and one spontaneous tumor from hamster. During an observation period of 21-28 days all cells formed rapidly growing tumors at the injection site. The critical cell dose varied in the different lines between 10-4 and 10-6 cells. Histological appearance of the neoplasias resembled that of the primary tumors. Two established lines from normal fibroblasts (3-T-3) and kidney (CV-1) failed to grow. Our results indicate that xenotransplantation in "nude" mice may serve as a simple in-vivo assay for testing the oncogenicity of tissue culture cells.
在先天性无胸腺小鼠突变体“裸鼠”中成功进行了5种不同的已建立肿瘤细胞系的异种移植。这些肿瘤包括一种人类宫颈癌（HeLa）、一种伯基特淋巴瘤，以及两种大鼠多瘤病毒诱导的肿瘤和一种仓鼠自发肿瘤。在21至28天的观察期内，所有细胞在注射部位均形成了快速生长的肿瘤。不同细胞系的临界细胞剂量在10^-4至10^-6个细胞之间变化。肿瘤的组织学外观与原发肿瘤相似。两种来自正常成纤维细胞（3-T-3）和肾脏（CV-1）的已建立细胞系未能生长。我们的结果表明，在“裸鼠”中进行异种移植可作为一种简单的体内试验，用于测试组织培养细胞的致癌性。
