Parvez Suhel, Pandey Suwarna, Ali Mehboob, Raisuddin Sheikh
Department of Neurology, University of Magdeburg, Leipziger Str. 44, D-39120 Magdeburg, Germany.
Sci Total Environ. 2006 Sep 15;368(2-3):627-36. doi: 10.1016/j.scitotenv.2006.04.011. Epub 2006 Jun 5.
The present investigation was carried out by sampling water, sediment and fish during a fish-kill episode at Panipat (Haryana, India), and again sampling at the same site was conducted after a gap of two months. During the second sampling no fish-kill was observed and the water was relatively less turbid and clear. Antioxidant profile and lipid peroxidation (LPO) in fish tissues were studied. Analysis was also carried out on the physico-chemical characteristics of water samples along with heavy metal and pesticide analysis in water and sediment samples during and after the episode. Dissolved oxygen level was substantially low during the fish-kill episode. Heavy metals (copper and chromium) and pesticides like BHC (Benzene hexachloride), DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane) were also detected during the episode in water and sediment samples. Various oxidative stress biomarkers in liver, kidney and gill tissues in the Indian freshwater fish Wallago attu (Bl. and Sch.) collected from the site were investigated. The levels of reduced glutathione and non-protein thiol were significantly (P<0.001) higher in the liver of Wallago attu collected from Panipat after the fish-kill episode. Ascorbic acid levels in all the tissues did not change significantly after the episode. The LPO in liver, kidney and gills was significantly low (P<0.01-0.001) in all tissues of fish collected after the fish-kill episode. The protein carbonyl levels were significantly low (P<0.05-0.01) in all the fish organs sampled after the fish-kill episode. The findings suggest that industrial effluent may result in the massive loss of a commercial commodity. The simultaneous measurement of the physicochemical parameters of the water samples showed a good correlation between the biomarkers responses and the environmental chemical stress conditions.
本研究在印度哈里亚纳邦潘尼帕特发生鱼类死亡事件期间对水、沉积物和鱼类进行了采样，并在两个月后于同一地点再次采样。第二次采样时未观察到鱼类死亡，且水体相对较清，浊度较低。研究了鱼类组织中的抗氧化概况和脂质过氧化（LPO）情况。还对事件期间及之后水样的理化特性进行了分析，并对水和沉积物样本中的重金属及农药进行了分析。鱼类死亡事件期间溶解氧水平极低。事件期间在水和沉积物样本中还检测到了重金属（铜和铬）以及农药，如六六六（六氯环己烷）、滴滴涕（1,1,1-三氯-2,2-双（对氯苯基）乙烷）。对从该地点采集的印度淡水鱼怀氏印度鲶（Bl. and Sch.）肝脏、肾脏和鳃组织中的各种氧化应激生物标志物进行了研究。鱼类死亡事件后从潘尼帕特采集的怀氏印度鲶肝脏中还原型谷胱甘肽和非蛋白硫醇水平显著升高（P<0.001）。事件后所有组织中的抗坏血酸水平均无显著变化。鱼类死亡事件后采集的鱼所有组织中肝脏、肾脏和鳃的脂质过氧化水平均显著降低（P<0.01 - 0.001）。鱼类死亡事件后采集的所有鱼器官中蛋白质羰基水平均显著降低（P<0.05 - 0.01）。研究结果表明，工业废水可能导致一种商业商品的大量损失。对水样理化参数的同步测量表明，生物标志物反应与环境化学应激条件之间具有良好的相关性。
