Chang Pi-Ling, Blair Harry C, Zhao Xiaochu, Chien Yi-wen, Chen Dungtsa, Tilden Arabella B, Chang Zhijie, Cao Xu, Faye-Petersen Ona Marie, Hicks Patricia
Department of Nutrition Sciences, University of Alabama, 35294-3360, USA.
Connect Tissue Res. 2006;47(2):67-76. doi: 10.1080/03008200600584074.
To better understand the potential use of fetal marrow stromal cells (MSCs) in bone tissue engineering, we compared the ability of these cells with those of adult MSCs with respect to osteoblasts differentiation in the presence or absence of glucocorticoids. Cells were grown for 3-4 weeks in basal medium or supplemented with 100 nM dexamethasone (DEX, a synthetic glucocorticoid analog) or with 50 microM L-ascorbate and 10 mM glycerol-2-phosphate (AS+GP) or with AS+GP+DEX. At various time points in culture, the following parameters were compared between fetal and adult MSCs: cell morphology, cell proliferation, alkaline phosphatase activity, calcium (45Ca) uptake, von Kossa staining, and glucocorticoids receptor expression were analyzed. Compared with adult MSCs, fetal cells showed a less dramatic change to cuboidal morphology in DEX-containing media. Fetal MSCs in all media conditions showed higher proliferation rates and lower alkaline phosphatase activities (p < 0.001) than adult cells. Both fetal and adult MSCs responded similarly in DEX-containing media with respect to suppressing cell proliferation, stimulating alkaline phosphatase activity, and consistently accumulating calcium (usually higher in fetal cells) with subsequent formation of mineralized matrix when compared with cells cultured in AS+GP. Our findings further implicate the requirement of glucocorticoids in osteogenesis. In conclusion, compared with adult MSCs, fetal cells showed greater ability in sustaining cell proliferation and calcium uptake suggesting that they may be useful for bone tissue repair.
为了更好地理解胎儿骨髓间充质干细胞（MSCs）在骨组织工程中的潜在用途，我们比较了这些细胞与成人MSCs在有或没有糖皮质激素存在的情况下成骨细胞分化的能力。细胞在基础培养基中培养3 - 4周，或添加100 nM地塞米松（DEX，一种合成糖皮质激素类似物），或添加50 microM L - 抗坏血酸和10 mM甘油 - 2 - 磷酸（AS + GP），或添加AS + GP + DEX。在培养的不同时间点，比较胎儿和成人MSCs之间的以下参数：细胞形态、细胞增殖、碱性磷酸酶活性、钙（45Ca）摄取、冯·科萨染色，并分析糖皮质激素受体表达。与成人MSCs相比，胎儿细胞在含DEX的培养基中向立方形形态的变化较小。在所有培养基条件下，胎儿MSCs的增殖率均高于成人细胞，碱性磷酸酶活性低于成人细胞（p < 0.001）。与在AS + GP中培养的细胞相比，胎儿和成人MSCs在含DEX的培养基中对抑制细胞增殖、刺激碱性磷酸酶活性以及持续积累钙（通常胎儿细胞中更高）并随后形成矿化基质的反应相似。我们的研究结果进一步表明糖皮质激素在骨生成中的必要性。总之，与成人MSCs相比，胎儿细胞在维持细胞增殖和钙摄取方面表现出更强的能力，表明它们可能对骨组织修复有用。
