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二氧化硅-庆大霉素纳米杂化物对人成骨样SaOS-2细胞成骨分化的影响

Effects of silica-gentamicin nanohybrids on osteogenic differentiation of human osteoblast-like SaOS-2 cells.

作者信息

He Wei, Mosselhy Dina A, Zheng Yudong, Feng Qingling, Li Xiaoning, Yang Xing, Yue Lina, Hannula Simo-Pekka

机构信息

School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, People's Republic of China.

Department of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, Espoo, Finland.

出版信息

Int J Nanomedicine. 2018 Feb 9;13:877-893. doi: 10.2147/IJN.S147849. eCollection 2018.

DOI:10.2147/IJN.S147849
PMID:29445277
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5810519/
Abstract

INTRODUCTION

In recent years, there has been an increasing interest in silica (SiO) nanoparticles (NPs) as drug delivery systems. This interest is mainly attributed to the ease of their surface functionalization for drug loading. In orthopedic applications, gentamicin-loaded SiO NPs (nanohybrids) are frequently utilized for their prolonged antibacterial effects. Therefore, the possible adverse effects of SiO-gentamicin nanohybrids on osteogenesis of bone-related cells should be thoroughly investigated to ensure safe applications.

MATERIALS AND METHODS

The effects of SiO-gentamicin nanohybrids on the cell viability and osteogenic differentiation of human osteoblast-like SaOS-2 cells were investigated, together with native SiO NPs and free gentamicin.

RESULTS

The results of Cell Count Kit-8 (CCK-8) assay show that both SiO-gentamicin nanohybrids and native SiO NPs reduce cell viability of SaOS-2 cells in a dose-dependent manner. Regarding osteogenesis, SiO-gentamicin nanohybrids and native SiO NPs at the concentration range of 31.25-125 μg/mL do not influence the osteogenic differentiation capacity of SaOS-2 cells. At a high concentration (250 μg/mL), both materials induce a lower expression of alkaline phosphatase (ALP) but an enhanced mineralization. Free gentamicin at concentrations of 6.26 and 9.65 μg/mL does not significantly influence the cell viability and osteogenic differentiation capacity of SaOS-2 cells.

CONCLUSIONS

The results of this study suggest that both SiO-gentamicin nanohybrids and SiO NPs show cytotoxic effects to SaOS-2 cells. Further investigation on the effects of SiO-gentamicin nanohybrids on the behaviors of stem cells or other regular osteoblasts should be conducted to make a full evaluation of the safety of SiO-gentamicin nanohybrids in orthopedic applications.

摘要

引言

近年来，作为药物递送系统的二氧化硅（SiO）纳米颗粒（NPs）受到越来越多的关注。这种关注主要归因于其表面易于功能化以负载药物。在骨科应用中，负载庆大霉素的SiO NPs（纳米杂化物）因其延长的抗菌作用而经常被使用。因此，应彻底研究SiO-庆大霉素纳米杂化物对骨相关细胞成骨作用的可能不良反应，以确保安全应用。

材料与方法

研究了SiO-庆大霉素纳米杂化物对人成骨样SaOS-2细胞的细胞活力和成骨分化的影响，同时研究了天然SiO NPs和游离庆大霉素的影响。

结果

细胞计数试剂盒-8（CCK-8）检测结果表明，SiO-庆大霉素纳米杂化物和天然SiO NPs均以剂量依赖性方式降低SaOS-2细胞的细胞活力。关于成骨作用，浓度范围为31.25-125μg/mL的SiO-庆大霉素纳米杂化物和天然SiO NPs不影响SaOS-2细胞的成骨分化能力。在高浓度（250μg/mL）下，两种材料均诱导碱性磷酸酶（ALP）表达降低，但矿化增强。浓度为6.26和9.65μg/mL的游离庆大霉素对SaOS-2细胞的细胞活力和成骨分化能力没有显著影响。

结论

本研究结果表明，SiO-庆大霉素纳米杂化物和SiO NPs均对SaOS-2细胞具有细胞毒性作用。应进一步研究SiO-庆大霉素纳米杂化物对干细胞或其他正常成骨细胞行为的影响，以全面评估SiO-庆大霉素纳米杂化物在骨科应用中的安全性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/7be5502c01c7/ijn-13-877Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/d3f9fcb7c40a/ijn-13-877Fig1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/7be5502c01c7/ijn-13-877Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/d3f9fcb7c40a/ijn-13-877Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/e3166633ccff/ijn-13-877Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/512ecab0b301/ijn-13-877Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/c929a66a0dd3/ijn-13-877Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/4d9c98cc3c73/ijn-13-877Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/bc620d1a86db/ijn-13-877Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/a83e8b8e90b0/ijn-13-877Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/b92b8307efa8/ijn-13-877Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/5810519/7be5502c01c7/ijn-13-877Fig9.jpg

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