• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
The expression, purification, crystallization and preliminary X-ray analysis of a subcomplex of the peripheral stalk of ATP synthase from bovine mitochondria.牛线粒体ATP合酶外周柄亚复合物的表达、纯化、结晶及初步X射线分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jun 1;62(Pt 6):530-3. doi: 10.1107/S1744309106015338. Epub 2006 May 5.
2
On the structure of the stator of the mitochondrial ATP synthase.关于线粒体ATP合酶定子的结构。
EMBO J. 2006 Jun 21;25(12):2911-8. doi: 10.1038/sj.emboj.7601177. Epub 2006 Jun 8.
3
The peripheral stalk of the mitochondrial ATP synthase.线粒体ATP合酶的外周柄。
Biochim Biophys Acta. 2006 May-Jun;1757(5-6):286-96. doi: 10.1016/j.bbabio.2006.01.001. Epub 2006 Jan 26.
4
ATP synthase: subunit-subunit interactions in the stator stalk.ATP合酶：定子柄中的亚基-亚基相互作用
Biochim Biophys Acta. 2006 Sep-Oct;1757(9-10):1162-70. doi: 10.1016/j.bbabio.2006.04.007. Epub 2006 Apr 19.
5
Crystallization and preliminary crystallographic studies of the mitochondrial F1-ATPase from the yeast Saccharomyces cerevisiae.酿酒酵母线粒体F1-ATP酶的结晶及初步晶体学研究
Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1441-4. doi: 10.1107/S0907444904012661. Epub 2004 Jul 21.
6
Structure of the mitochondrial ATP synthase by electron cryomicroscopy.通过电子冷冻显微镜观察线粒体ATP合酶的结构
EMBO J. 2003 Dec 1;22(23):6182-92. doi: 10.1093/emboj/cdg608.
7
Purification, crystallization, and properties of F1-ATPase complexes from the thermoalkaliphilic Bacillus sp. strain TA2.A1.嗜热嗜碱芽孢杆菌TA2.A1菌株F1-ATP酶复合物的纯化、结晶及性质
J Struct Biol. 2005 Nov;152(2):140-5. doi: 10.1016/j.jsb.2005.08.005. Epub 2005 Sep 16.
8
The structure of the membrane extrinsic region of bovine ATP synthase.牛三磷酸腺苷合酶膜外区域的结构。
Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21597-601. doi: 10.1073/pnas.0910365106. Epub 2009 Dec 7.
9
Structure of the F1-binding domain of the stator of bovine F1Fo-ATPase and how it binds an alpha-subunit.牛F1Fo - ATP合酶定子的F1结合结构域及其与α亚基的结合方式。
J Mol Biol. 2005 Aug 26;351(4):824-38. doi: 10.1016/j.jmb.2005.06.012.
10
Interactions of subunits Asa2, Asa4 and Asa7 in the peripheral stalk of the mitochondrial ATP synthase of the chlorophycean alga Polytomella sp.绿藻多鞭藻线粒体ATP合酶外周柄中亚基Asa2、Asa4和Asa7的相互作用
Biochim Biophys Acta. 2014 Jan;1837(1):1-13. doi: 10.1016/j.bbabio.2013.08.001. Epub 2013 Aug 9.

引用本文的文献

1
Trypanosoma brucei TbIF1 inhibits the essential F1-ATPase in the infectious form of the parasite.布氏锥虫TbIF1抑制该寄生虫感染形式下的关键F1-ATP酶。
PLoS Negl Trop Dis. 2017 Apr 17;11(4):e0005552. doi: 10.1371/journal.pntd.0005552. eCollection 2017 Apr.
2
The affinity purification and characterization of ATP synthase complexes from mitochondria.从线粒体中纯化和鉴定 ATP 合酶复合物。
Open Biol. 2013 Feb 13;3(2):120160. doi: 10.1098/rsob.120160.
3
Introduction of the chloroplast redox regulatory region in the yeast ATP synthase impairs cytochrome c oxidase.将叶绿体氧化还原调节区域引入酵母ATP合酶会损害细胞色素c氧化酶。
J Biol Chem. 2008 Nov 21;283(47):32937-43. doi: 10.1074/jbc.M805310200. Epub 2008 Sep 26.
4
Yeast cells depleted in Atp14p fail to assemble Atp6p within the ATP synthase and exhibit altered mitochondrial cristae morphology.Atp14p缺失的酵母细胞无法在ATP合酶内组装Atp6p，并表现出线粒体嵴形态的改变。
J Biol Chem. 2008 Apr 11;283(15):9749-58. doi: 10.1074/jbc.M800204200. Epub 2008 Feb 5.
5
Reevaluating the relationship between EPR spectra and enzyme structure for the iron sulfur clusters in NADH:quinone oxidoreductase.重新评估NADH：醌氧化还原酶中铁硫簇的电子顺磁共振光谱（EPR光谱）与酶结构之间的关系。
Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12720-5. doi: 10.1073/pnas.0705593104. Epub 2007 Jul 19.
6
Mitochondrial genome integrity mutations uncouple the yeast Saccharomyces cerevisiae ATP synthase.线粒体基因组完整性突变使酿酒酵母的ATP合酶解偶联。
J Biol Chem. 2007 Mar 16;282(11):8228-36. doi: 10.1074/jbc.M609635200. Epub 2007 Jan 23.
7
On the structure of the stator of the mitochondrial ATP synthase.关于线粒体ATP合酶定子的结构。
EMBO J. 2006 Jun 21;25(12):2911-8. doi: 10.1038/sj.emboj.7601177. Epub 2006 Jun 8.

本文引用的文献

1
Scaling and assessment of data quality.数据质量的评估与分级
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82. doi: 10.1107/S0907444905036693. Epub 2005 Dec 14.
2
The structure of bovine F1-ATPase inhibited by ADP and beryllium fluoride.被二磷酸腺苷（ADP）和氟化铍抑制的牛F1-ATP酶的结构
EMBO J. 2004 Jul 21;23(14):2734-44. doi: 10.1038/sj.emboj.7600293. Epub 2004 Jul 1.
3
Structure of bovine mitochondrial F(1)-ATPase with nucleotide bound to all three catalytic sites: implications for the mechanism of rotary catalysis.牛线粒体F1 - ATP酶的结构，其中核苷酸与所有三个催化位点结合：对旋转催化机制的启示
Cell. 2001 Aug 10;106(3):331-41. doi: 10.1016/s0092-8674(01)00452-4.
4
The structure of the central stalk in bovine F(1)-ATPase at 2.4 A resolution.牛F1 - ATP合酶中中央柄在2.4埃分辨率下的结构。
Nat Struct Biol. 2000 Nov;7(11):1055-61. doi: 10.1038/80981.
5
Molecular architecture of the rotary motor in ATP synthase.ATP合酶中旋转马达的分子结构
Science. 1999 Nov 26;286(5445):1700-5. doi: 10.1126/science.286.5445.1700.
6
ATP synthase--past and future.ATP合酶——过去与未来
Biochim Biophys Acta. 1998 Jun 10;1365(1-2):3-9. doi: 10.1016/s0005-2728(98)00066-8.
7
The F1F0-ATPase complex from bovine heart mitochondria: the molar ratio of the subunits in the stalk region linking the F1 and F0 domains.来自牛心线粒体的F1F0 - ATP酶复合体：连接F1和F0结构域的柄部区域中亚基的摩尔比。
Biochemistry. 1996 Sep 24;35(38):12640-6. doi: 10.1021/bi960969t.
8
Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels.大肠杆菌中蛋白质的过量生产：能使某些膜蛋白和球状蛋白高水平合成的突变宿主。
J Mol Biol. 1996 Jul 19;260(3):289-98. doi: 10.1006/jmbi.1996.0399.
9
Structure at 2.8 A resolution of F1-ATPase from bovine heart mitochondria.牛心线粒体F1-ATP酶2.8埃分辨率的结构
Nature. 1994 Aug 25;370(6491):621-8. doi: 10.1038/370621a0.
10
ATP synthase from bovine heart mitochondria. In vitro assembly of a stalk complex in the presence of F1-ATPase and in its absence.来自牛心线粒体的ATP合酶。在存在F1 - ATP酶和不存在F1 - ATP酶的情况下体外组装茎复合体。
J Mol Biol. 1994 Sep 30;242(4):408-21. doi: 10.1006/jmbi.1994.1591.

牛线粒体ATP合酶外周柄亚复合物的表达、纯化、结晶及初步X射线分析

The expression, purification, crystallization and preliminary X-ray analysis of a subcomplex of the peripheral stalk of ATP synthase from bovine mitochondria.

作者信息

Silvester Jocelyn A, Dickson Veronica Kane, Runswick Michael J, Leslie Andrew G W, Walker John E

机构信息

The Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, England.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jun 1;62(Pt 6):530-3. doi: 10.1107/S1744309106015338. Epub 2006 May 5.

DOI:10.1107/S1744309106015338
PMID:16754973
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2243082/
Abstract

A subcomplex of the peripheral stalk or stator domain of the ATP synthase from bovine mitochondria has been expressed to high levels in a soluble form in Escherichia coli. The subcomplex consists of residues 79-184 of subunit b, residues 1-124 of subunit d and the entire F6 subunit (76 residues). It has been purified and crystallized by vapour diffusion. The morphology and diffraction properties of the crystals of the subcomplex were improved by the presence of thioxane or 4-methylpyridine in the crystallization liquor. With a synchrotron-radiation source, these crystals diffracted to 2.8 A resolution. They belong to the monoclinic space group P2(1).

摘要

牛线粒体ATP合酶外周柄或定子结构域的一个亚复合物已在大肠杆菌中以可溶形式高水平表达。该亚复合物由b亚基的79 - 184位残基、d亚基的1 - 124位残基和整个F6亚基（76个残基）组成。它已通过气相扩散法进行了纯化和结晶。结晶液中存在噻嗯或4 - 甲基吡啶可改善该亚复合物晶体的形态和衍射特性。利用同步辐射源，这些晶体的衍射分辨率达到了2.8埃。它们属于单斜空间群P2(1)。