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ATP Synthesis by Rotary Catalysis (Nobel lecture).通过旋转催化进行ATP合成（诺贝尔演讲）。
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A model for the cooperative free energy transduction and kinetics of ATP hydrolysis by F1-ATPase.一种关于F1-ATP酶协同自由能转导及ATP水解动力学的模型。
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Partial resolution of the enzymes catalyzing oxidative phosphorylation. I. Purification and properties of soluble dinitrophenol-stimulated adenosine triphosphatase.催化氧化磷酸化的酶的部分解析。I. 可溶性二硝基苯酚刺激的三磷酸腺苷酶的纯化及性质
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The structure of bovine F1-ATPase in complex with its regulatory protein IF1.牛F1-ATP酶与其调节蛋白IF1结合时的结构。
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被二磷酸腺苷（ADP）和氟化铍抑制的牛F1-ATP酶的结构

The structure of bovine F1-ATPase inhibited by ADP and beryllium fluoride.

作者信息

Kagawa Reiko, Montgomery Martin G, Braig Kerstin, Leslie Andrew G W, Walker John E

机构信息

The Medical Research Council Dunn Human Nutrition Unit, Cambridge, UK.

出版信息

EMBO J. 2004 Jul 21;23(14):2734-44. doi: 10.1038/sj.emboj.7600293. Epub 2004 Jul 1.

DOI:10.1038/sj.emboj.7600293
PMID:15229653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC514953/
Abstract

The structure of bovine F1-ATPase inhibited with ADP and beryllium fluoride at 2.0 angstroms resolution contains two ADP.BeF3- complexes mimicking ATP, bound in the catalytic sites of the beta(TP) and beta(DP) subunits. Except for a 1 angstrom shift in the guanidinium of alphaArg373, the conformations of catalytic side chains are very similar in both sites. However, the ordered water molecule that carries out nucleophilic attack on the gamma-phosphate of ATP during hydrolysis is 2.6 angstroms from the beryllium in the beta(DP) subunit and 3.8 angstroms away in the beta(TP) subunit, strongly indicating that the beta(DP) subunit is the catalytically active conformation. In the structure of F1-ATPase with five bound ADP molecules (three in alpha-subunits, one each in the beta(TP) and beta(DP) subunits), which has also been determined, the conformation of alphaArg373 suggests that it senses the presence (or absence) of the gamma-phosphate of ATP. Two catalytic schemes are discussed concerning the various structures of bovine F1-ATPase.

摘要

在2.0埃分辨率下，被二磷酸腺苷（ADP）和氟化铍抑制的牛F1 - ATP合酶结构包含两个模拟三磷酸腺苷（ATP）的ADP·BeF₃⁻复合物，它们结合在β(TP)和β(DP)亚基的催化位点上。除了αArg373的胍基有1埃的位移外，两个位点催化侧链的构象非常相似。然而，在水解过程中对ATP的γ - 磷酸进行亲核攻击的有序水分子，在β(DP)亚基中距离铍为2.6埃，在β(TP)亚基中距离铍为3.8埃，这有力地表明β(DP)亚基是具有催化活性的构象。在已确定的结合有五个ADP分子（三个在α亚基中，一个在β(TP)亚基中，一个在β(DP)亚基中）的F1 - ATP合酶结构中，αArg373的构象表明它能感知ATP的γ - 磷酸的存在（或不存在）。讨论了关于牛F1 - ATP合酶各种结构的两种催化机制。