Howard P L, Collins C C, Heintz N H
Department of Pathology, University of Vermont, Burlington.
Transfusion. 1991 Jun;31(5):441-2. doi: 10.1046/j.1537-2995.1991.31591263200.x.
The assignment of paternity when the alleged father has died is now possible by use of a variety of allele-specific oligonucleotides after amplification of genomic DNA by the polymerase chain reaction (PCR). Issues relating to the inheritance of estates may be decided on fact rather than allegation. PCR-based genotyping of DQ alpha haplotypes from paraffin-embedded tissue of the deceased was used to prove non-paternity in the case reported here. Because the child was female, it was also possible to confirm the exclusion by using a second polymorphic site located in the factor VIII gene on the X chromosome.
当被指控的父亲去世后，现在可以通过聚合酶链反应（PCR）扩增基因组DNA后使用各种等位基因特异性寡核苷酸来确定父子关系。与遗产继承相关的问题可以根据事实而非指控来决定。在此报道的案例中，对死者石蜡包埋组织的DQα单倍型进行基于PCR的基因分型以证明不存在父子关系。由于孩子是女性，还可以通过位于X染色体上的凝血因子VIII基因中的第二个多态性位点来确认排除。
