Mulhare P, McQuillen E, Collins C, Heintz N, Howard P
Department of Pathology, University of Vermont College of Medicine, Burlington 05405.
Am J Forensic Med Pathol. 1991 Jun;12(2):157-60. doi: 10.1097/00000433-199106000-00013.
The 2-year-old daughter of two farm laborers was reported missing while the farm owner was harvesting corn. Unidentifiable tissues and body parts were subsequently found admixed with silage. Samples of blood collected from the parents of the missing child as well as portions of the tissue recovered from the silage were subjected to analysis of DNA polymorphisms with probes usually used to identify paternity. In addition, allele-specific oligonucleotides were used to detect DNA polymorphism at the DQ alpha locus following DNA amplification using the polymerase chain reaction. In this case, the DNA results established that the tissue recovered from the silage was of human origin and confirmed the probable parentage of the two farm laborers.
据报道，在农场主收割玉米时，两名农场工人两岁的女儿失踪了。随后，在青贮饲料中发现了无法辨认的人体组织和身体部位。从失踪儿童的父母身上采集了血样，并对从青贮饲料中找到的部分组织，用通常用于亲子鉴定的探针进行了DNA多态性分析。此外，在使用聚合酶链反应进行DNA扩增后，用等位基因特异性寡核苷酸检测了DQα位点的DNA多态性。在这个案例中，DNA检测结果证实，从青贮饲料中找到的组织来自人类，并确认了这两名农场工人是其可能的父母。
