黄粉虫（黄粉甲）中萤火虫荧光素酶同源基因的克隆与鉴定
Cloning and characterization of the homologous genes of firefly luciferase in the mealworm beetle, Tenebrio molitor.
作者信息
Oba Y, Sato M, Inouye S
机构信息
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
出版信息
Insect Mol Biol. 2006 Jun;15(3):293-9. doi: 10.1111/j.1365-2583.2006.00646.x.
Three homologous genes of firefly luciferase were cloned from the non-luminous beetle Tenebrio molitor. Three gene products for homologues, TmLL-1, TmLL-2 and TmLL-3, showed fatty acyl-coenzyme A (acyl-CoA) synthetic activity, but not luciferase activity with firefly luciferin. The transcripts were detected through the developmental stages in T. molitor. These results suggested that firefly luciferase was evolved from a fatty acyl-coenzyme A synthetase by gene duplications in the insect.
从非发光甲虫黄粉虫中克隆出了萤火虫荧光素酶的三个同源基因。三个同源物的基因产物TmLL-1、TmLL-2和TmLL-3表现出脂肪酰辅酶A（酰基辅酶A）合成活性，但对萤火虫荧光素没有荧光素酶活性。在黄粉虫的发育阶段检测到了这些转录本。这些结果表明，萤火虫荧光素酶是昆虫中通过基因复制从脂肪酰辅酶A合成酶进化而来的。
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