Jinwal U K, Zakharkin S O, Litvinova O V, Jain S, Benes Helen
Department of Neurobiology and Developmental Sciences, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Insect Mol Biol. 2006 Jun;15(3):301-11. doi: 10.1111/j.1365-2583.2006.00644.x.
A portion of the 5'-flanking region of the female-specific hexamerin gene, Hex-1.2, from the mosquito Ochlerotatus atropalpus was used to drive expression of the luciferase reporter gene in Drosophila melanogaster. The proximal 0.7 kb of 5'-flanking DNA were sufficient to partially repress reporter gene activity in males and to drive tissue- and stage-specific expression comparable with that of the endogenous O. atropalpus Hex-1.2 gene. The Drosophila doublesex transcription factor (DSX), expressed in Escherichia coli, bound putative DSX sites of the Hex-1.2 gene differentially in vitro. Blocking expression of the female isoform of the Doublesex transcription factor in transgenic female flies resulted in reduction of luciferase expression to levels comparable with those in males, suggesting that Doublesex could contribute to regulation of female-specific expression of the O. atropalpus Hex-1.2 gene.
来自致倦库蚊（Ochlerotatus atropalpus）的雌性特异性六聚体蛋白基因Hex-1.2的5'-侧翼区域的一部分，被用于驱动果蝇（Drosophila melanogaster）中荧光素酶报告基因的表达。5'-侧翼DNA近端的0.7 kb足以部分抑制雄性中的报告基因活性，并驱动与内源性致倦库蚊Hex-1.2基因相当的组织和阶段特异性表达。在大肠杆菌中表达的果蝇双性转录因子（DSX），在体外与Hex-1.2基因的假定DSX位点有不同的结合。在转基因雌性果蝇中阻断双性转录因子雌性异构体的表达，导致荧光素酶表达降低至与雄性相当的水平，这表明双性转录因子可能有助于调节致倦库蚊Hex-1.2基因的雌性特异性表达。
