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使用白光热透镜分光光度计测量的染料溶液吸收光谱。

Absorption spectra of dye solutions measured using a white light thermal lens spectrophotometer.

作者信息

Marcano O A, Ojeda J, Melikechi N

机构信息

Applied Optics Center, Delaware State University, Dover, Delaware 19901, USA.

出版信息

Appl Spectrosc. 2006 May;60(5):560-3. doi: 10.1366/000370206777412077.

DOI:10.1366/000370206777412077
PMID:16756708
Abstract

We describe a two-beam thermal lens spectrophotometer that uses a xenon lamp as the excitation source and a low power diode pumped Nd-YAG laser as the probe light. The white light from the xenon source is filtered using a variable interference filter producing a partial monochromatic light within the spectral range 400-700 nm and with a spectral resolution of 10 nm. We measure the thermal lens spectrum of a nonfluorescent dye (Malachite Green) and show that this spectrum reproduces its absorbance spectra measured by the usual transmission method. A comparison of the thermal lens and the absorbance spectra of a fluorescent dye (Rhodamine B) reveals substantial differences. These differences can yield important applications of the device for the characterization of fluorescent materials.

摘要

我们描述了一种双光束热透镜分光光度计,它使用氙灯作为激发源,低功率二极管泵浦的Nd-YAG激光器作为探测光。氙光源发出的白光通过可变干涉滤光片进行滤波,产生光谱范围在400 - 700 nm、光谱分辨率为10 nm的部分单色光。我们测量了一种非荧光染料(孔雀石绿)的热透镜光谱,并表明该光谱再现了通过常规透射法测量的其吸收光谱。荧光染料(罗丹明B)的热透镜光谱与吸收光谱的比较揭示了显著差异。这些差异可为该装置在荧光材料表征方面带来重要应用。

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