Xu Hai Jun, Liu Yan He, Yang Zhang Nv, Zhang Chuan Xi
Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
J Biochem Mol Biol. 2006 May 31;39(3):263-9. doi: 10.5483/bmbrep.2006.39.3.263.
In the genome of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus, open reading frame 39 (Ha39) is the only gene predicted to encode an RNA recognition protein. Computer analysis revealed that Ha39 homologues were found in 15 NPVs, but not in GVs. Its transcripts were detected from 3 through 72 hours post infection (h p.i.) using RT-PCR and Northern blot analysis. The protein was detected in infected-cell lysates from 6 h p.i. Western blot assay of ODV and BV preparations revealed that Ha39 encodes a structural protein associated with BVs.Additionally, immunofluorescence microscopy demonstrated that the protein was present within cytoplasm in virusinfected cells, but not in the nuclear region.
在棉铃虫单粒包埋核型多角体病毒的基因组中，开放阅读框39（Ha39）是唯一预测编码RNA识别蛋白的基因。计算机分析显示，在15种核型多角体病毒中发现了Ha39的同源物，但在颗粒体病毒中未发现。使用逆转录聚合酶链反应（RT-PCR）和Northern印迹分析，在感染后3至72小时（h p.i.）检测到其转录本。在感染后6小时的感染细胞裂解物中检测到该蛋白。对病毒粒子（ODV）和芽生病毒（BV）制剂的蛋白质印迹分析表明，Ha39编码一种与BV相关的结构蛋白。此外，免疫荧光显微镜检查表明，该蛋白存在于病毒感染细胞的细胞质中，但不存在于核区域。
