Kishi Katsuyuki, Kitagawa Emiko, Onikura Norio, Nakamura Akiko, Iwahashi Hitoshi
Japan Pulp & Paper Research Institute, Inc., Tokodai 5-13-11, Tsukuba, Ibaraki 300-2635, Japan.
Genomics. 2006 Aug;88(2):241-51. doi: 10.1016/j.ygeno.2006.03.023. Epub 2006 Jun 6.
Gene profiling of Japanese medaka (Oryzias latipes) was performed using an oligonucleotide DNA microarray representing 22,587 TIGR O. latipes gene indices (OLGIs). The average correlation coefficients for gene expression between individual mature fish were high (>0.95) for both female and male, indicating that the physiological status of medaka is highly reproducible under prescribed growth conditions. Of the 22,587 OLGIs, 2575 showed significant differences in expression between female and male. Exposure to 17beta-estradiol (E2) revealed 381 E2-responsive OLGIs in male medaka. Feminization and male-dysfunction factors of the E2-treated males calculated using the combination of Pearson correlation coefficient and Euclidean distances indicate that E2 treatment "weakly feminized" male medaka, while male physiological functions were not significantly disrupted. This study demonstrates the possibility of using medaka microarrays to estimate the overall effects of hormonally active chemicals.
利用代表22,587个稻属基因索引（OLGIs）的寡核苷酸DNA微阵列对日本青鳉（Oryzias latipes）进行基因谱分析。雌性和雄性个体成熟鱼之间基因表达的平均相关系数都很高（>0.95），这表明在规定的生长条件下，青鳉的生理状态具有高度可重复性。在22,587个OLGIs中，有2575个在雌性和雄性之间表现出显著的表达差异。暴露于17β-雌二醇（E2）后，在雄性青鳉中发现了381个E2反应性OLGIs。使用皮尔逊相关系数和欧几里得距离相结合的方法计算E2处理雄性的雌性化和雄性功能障碍因子，结果表明E2处理使雄性青鳉“轻微雌性化”，而雄性生理功能没有受到显著破坏。这项研究证明了使用青鳉微阵列来评估激素活性化学物质总体影响的可能性。
