Nardo B, Puviani L, Caraceni P, Pacilè V, Bertelli R, Beltempo P, Cavallari G, Chieco P, Pariali M, Pertosa A M, Angiolini G, Domenicali M, Neri F, Tsivian M, Bernardi M, Cavallari A
Department of Surgery and Transplantations, S. Orsola-Malpighi Hospital, University of Bologna, Italy.
Transplant Proc. 2006 May;38(4):1187-9. doi: 10.1016/j.transproceed.2006.03.056.
Optimization of the conditions for regeneration of the native diseased liver is a major goal in patients with acute liver failure. This study sought to determine whether portal vein arterialization (PVA), which increases the oxygen supply to the liver, was protective in a rat model of liver failure.
At 24 hours after CCl(4) intoxication, Sprague-Dawley rats (six per group) were assigned to receive PVA or as controls. We determined blood tests, histology, and 10-day survivals. Hepatocyte regeneration was assessed by the mitotic index and bromodeoxyuridine (BrdU) incorporation.
Serum transaminases were significantly lower in PVA-treated rats than in control animals: liver necrosis resolved rapidly after PVA. The BrdU staining and mitotic index were severalfold higher among PVA-treated than in untreated rats. Survival was 100% among rats with PVA and 40% in untreated animals (P < .01).
PVA led to resolution of CCl(4)-induced massive liver necrosis in the rat. This effect was probably mediated by activation of rapid and extensive hepatocyte regeneration. PVA might provide a novel, alternative approach to treat acute liver failure.
优化天然病变肝脏的再生条件是急性肝衰竭患者的主要目标。本研究旨在确定增加肝脏氧供应的门静脉动脉化（PVA）在肝衰竭大鼠模型中是否具有保护作用。
在四氯化碳中毒24小时后，将Sprague-Dawley大鼠（每组6只）分为接受PVA组或作为对照组。我们测定了血液检查、组织学和10天生存率。通过有丝分裂指数和溴脱氧尿苷（BrdU）掺入评估肝细胞再生。
PVA治疗的大鼠血清转氨酶显著低于对照动物：PVA后肝坏死迅速消退。PVA治疗的大鼠中BrdU染色和有丝分裂指数比未治疗的大鼠高几倍。PVA大鼠的生存率为100%，未治疗动物的生存率为40%（P <.01）。
PVA导致大鼠四氯化碳诱导的大规模肝坏死消退。这种作用可能是由快速和广泛的肝细胞再生激活介导的。PVA可能为治疗急性肝衰竭提供一种新的替代方法。
