门静脉动脉化治疗大鼠肝切除术后急性肝衰竭
Portal vein arterialization for the treatment of post resection acute liver failure in the rat.
作者信息
Nardo B, Puviani L, Caraceni P, Pacilè V, Bertelli R, Beltempo P, Cavallari G, Chieco P, Pariali M, Pertosa A M, Angiolini G, Domenicali M, Neri F, Prezzi D, Tsivian M, Bernardi M, Cavallari A
机构信息
Department of Surgery and Transplantations, S. Orsola-Malpighi Hospital, University of Bologna, Italy.
出版信息
Transplant Proc. 2006 May;38(4):1185-6. doi: 10.1016/j.transproceed.2006.03.061.
INTRODUCTION
Hyperoxygenation of the liver has been suggested to improve its regenerative capacity. Thus, this study sought to determine whether an additional supply of oxygenated blood delivered by portal vein arterialization (PVA) was protective against acute liver failure induced by hepatectomy.
METHODS
Sprague-Dawley rats (six per each group) were divided to either undergo PVA or be untreated after extended hepatectomy. Liver injury was evaluated by the serum alanine aminotransferase (ALT) levels. Hepatocyte regeneration was assessed by calculating the mitotic index and bromodeoxyuridine staining. The 10-day survival was assessed in separate experimental groups.
RESULTS
The pO(2) in portal blood increased significantly following PVA. Serum ALT levels were significantly reduced in arterialized versus nonarterialized rats. PVA promotes liver regeneration. Finally, PVA significantly improved host survival compared to the controls: 90% versus 30%, respectively.
CONCLUSION
These data suggested that an additional supply of arterial oxygenated blood through PVA promoted a rapid regeneration, leading to a faster restoration of liver mass after partial hepatectomy in rats. Thus, PVA may represent a novel tool to optimize hepatocyte regeneration.
引言
肝脏的过度氧合已被认为可提高其再生能力。因此，本研究旨在确定通过门静脉动脉化（PVA）额外供应含氧血液是否对肝切除诱导的急性肝衰竭具有保护作用。
方法
将Sprague-Dawley大鼠（每组6只）分为两组，一组进行PVA，另一组在扩大肝切除术后不进行处理。通过血清丙氨酸氨基转移酶（ALT）水平评估肝损伤。通过计算有丝分裂指数和溴脱氧尿苷染色评估肝细胞再生。在单独的实验组中评估10天生存率。
结果
PVA后门静脉血中的pO(2)显著升高。与未动脉化的大鼠相比，动脉化大鼠的血清ALT水平显著降低。PVA促进肝脏再生。最后，与对照组相比，PVA显著提高了宿主生存率：分别为90%和30%。
结论
这些数据表明，通过PVA额外供应动脉含氧血液促进了快速再生，导致大鼠部分肝切除后肝质量更快恢复。因此，PVA可能是一种优化肝细胞再生的新工具。
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