Lee Jeong Wook, Lee Sang Yup, Song Hyohak, Yoo Jong-Shin
Department of Chemical & Biomolecular Engineering, Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
Proteomics. 2006 Jun;6(12):3550-66. doi: 10.1002/pmic.200500837.
Mannheimia succiniciproducens MBEL55E isolated from bovine rumen is an industrially important bacterium as an efficient succinic acid producer. Recently, its full genome sequence was determined. In the present study, we analyzed the M. succiniciproducens proteome based on the genome information using 2-DE and MS. We established proteome reference map of M. succiniciproducens by analyzing whole cellular proteins, membrane proteins, and secreted proteins. More than 200 proteins were identified and characterized by MS/MS supported by various bioinformatic tools. The presence of proteins previously annotated as hypothetical proteins or proteins having putative functions were also confirmed. Based on the proteome reference map, cells in the different growth phases were analyzed at the proteome level. Comparative proteome profiling revealed valuable information to understand physiological changes during growth, and subsequently suggested target genes to be manipulated for the strain improvement.
从牛瘤胃中分离出的产琥珀酸曼氏杆菌MBEL55E作为一种高效的琥珀酸生产者，是一种具有重要工业价值的细菌。最近，其全基因组序列已被测定。在本研究中，我们基于基因组信息，利用二维电泳（2-DE）和质谱（MS）分析了产琥珀酸曼氏杆菌的蛋白质组。通过分析全细胞蛋白、膜蛋白和分泌蛋白，我们建立了产琥珀酸曼氏杆菌的蛋白质组参考图谱。通过各种生物信息学工具支持的串联质谱（MS/MS）鉴定并表征了200多种蛋白质。先前被注释为假设蛋白或具有推定功能的蛋白质的存在也得到了证实。基于蛋白质组参考图谱，在蛋白质组水平上分析了不同生长阶段的细胞。比较蛋白质组分析揭示了有助于理解生长过程中生理变化的有价值信息，并随后提出了为菌株改良而需操纵的靶基因。
