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大肠杆菌对长链脂肪酸的蛋白质组水平响应以及脂肪酸诱导型启动子在蛋白质生产中的应用。

Proteome-level responses of Escherichia coli to long-chain fatty acids and use of fatty acid inducible promoter in protein production.

作者信息

Han Mee-Jung, Lee Jeong Wook, Lee Sang Yup, Yoo Jong Shin

机构信息

Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), Yuseong-gu, Daejeon 305-701, South Korea.

出版信息

J Biomed Biotechnol. 2008;2008:735101. doi: 10.1155/2008/735101.

DOI:10.1155/2008/735101
PMID:18317523
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2218904/
Abstract

In Escherichia coli, a long-chain acyl-CoA is a regulatory signal that modulates gene expression through its binding to a transcription factor FadR. In this study, comparative proteomic analysis of E. coli in the presence of glucose and oleic acid was performed to understand cell physiology in response to oleic acid. Among total of 52 proteins showing altered expression levels with oleic acid presence, 9 proteins including AldA, Cdd, FadA, FadB, FadL, MalE, RbsB, Udp, and YccU were newly synthesized. Among the genes that were induced by oleic acid, the promoter of the aldA gene was used for the production of a green fluorescent protein (GFP). Analysis of fluorescence intensities and confocal microscopic images revealed that soluble GFP was highly expressed under the control of the aldA promoter. These results suggest that proteomics is playing an important role not only in biological research but also in various biotechnological applications.

摘要

在大肠杆菌中，长链酰基辅酶A是一种调节信号，它通过与转录因子FadR结合来调节基因表达。在本研究中，对存在葡萄糖和油酸情况下的大肠杆菌进行了比较蛋白质组学分析，以了解细胞对油酸的生理反应。在因油酸存在而表达水平发生改变的总共52种蛋白质中，包括AldA、Cdd、FadA、FadB、FadL、MalE、RbsB、Udp和YccU在内的9种蛋白质是新合成的。在由油酸诱导的基因中，aldA基因的启动子被用于生产绿色荧光蛋白（GFP）。荧光强度分析和共聚焦显微镜图像显示，可溶性GFP在aldA启动子的控制下高度表达。这些结果表明，蛋白质组学不仅在生物学研究中，而且在各种生物技术应用中都发挥着重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/7b244b3dba47/JBB2008-735101.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/bf695ccfb4ec/JBB2008-735101.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/7c790948d0a6/JBB2008-735101.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/0cc531d29536/JBB2008-735101.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/881737a8d55a/JBB2008-735101.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/6a16d690daac/JBB2008-735101.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/7b244b3dba47/JBB2008-735101.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/bf695ccfb4ec/JBB2008-735101.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/7c790948d0a6/JBB2008-735101.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/0cc531d29536/JBB2008-735101.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/881737a8d55a/JBB2008-735101.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/6a16d690daac/JBB2008-735101.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e76/2218904/7b244b3dba47/JBB2008-735101.006.jpg

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