Konstantinova N A, Karandashov E N
Department of Experimental and Theoretical Physics, Russian State Medical University, Moscow.
Bull Exp Biol Med. 2005 Nov;140(5):585-7. doi: 10.1007/s10517-006-0031-5.
For studies of the charge characteristics of human erythrocytes loaded with cryoglobulins the cells should be stored in Alsever solution with 0.1% sodium azide no longer than 1 week after blood collection. Erythrocytes should be loaded with cryoglobulins by incubation during at least 4 h, the final concentration of cryoproteins in the solution being no lower than 30 microg/ml.
对于加载冷球蛋白的人红细胞电荷特性的研究,细胞应在采集血液后于含有0.1%叠氮化钠的阿氏液中保存,保存时间不超过1周。红细胞应通过至少4小时的孵育加载冷球蛋白,溶液中冷冻保护蛋白的最终浓度不低于30微克/毫升。