Fowler A J, Toner M
Mechanical Engineering Department, University of Massachusetts, Dartmouth 02747, USA.
Ann N Y Acad Sci. 1998 Sep 11;858:245-52. doi: 10.1111/j.1749-6632.1998.tb10158.x.
This paper reports on the successful recovery of rapidly frozen unprotected erythrocytes by vitrification of the intracellular solution with a laser pulse prior to thawing. Erythrocytes that were frozen at 10,000 degrees C/min exhibited 100% hemolysis when thawed unless they were first irradiated by a 7 ns. laser pulse that selectively targeted the intracellular ice so that it was melted and resolidified into a glass phase. Up to 80% of the cells treated in this way remained intact after thawing. Wright's staining confirmed a healthy cell morphology and the retention of hemoglobin in the laser treated cells. While it is well known that small amounts of intracellular ice can be tolerated by cells, the findings of this study are the first to indicate that intracellular ice may be innocuous even when formed in substantial quantities provided that crystal growth and coalescence can somehow be avoided during warming.
本文报道了在解冻前通过激光脉冲使细胞内溶液玻璃化，从而成功复苏快速冷冻的未保护红细胞。以每分钟10000摄氏度冷冻的红细胞解冻时会出现100%溶血，除非先用7纳秒激光脉冲进行照射，该脉冲选择性地作用于细胞内冰，使其融化并重新凝固成玻璃态。以这种方式处理的细胞解冻后高达80%保持完整。瑞氏染色证实激光处理后的细胞形态健康且血红蛋白得以保留。虽然众所周知细胞能够耐受少量细胞内冰，但本研究结果首次表明，即使大量形成细胞内冰，只要在升温过程中能够以某种方式避免晶体生长和聚结，细胞内冰可能也是无害的。
