Li Jing, Li Zhong, Mo Bao-qing
Department of Nutrition and Food Hygiene, Nanjing Medical University, Nanjing 210029, China.
Wei Sheng Yan Jiu. 2006 Mar;35(2):184-6.
To study the role of ERK5 MAPK signaling transduction pathway in the inhibition of genistein to MDA-MB-231 breast cancer cells.
The inhibitive effects of genistein on MDA-MB-231 cells were observed with the MTT assay. The cell apoptosis was detected by flow cytometry. The ERK5 MAPK, Bax and Caspase3 protein expressions were detected by using western blot method.
genistein inhibited the cell proliferation of MDA-MB-231 cells and induced apoptosis. The protein expression of ERK5 decreased and those of Bax and Caspase3 increased with the dose of genistein.
Genistein can affect the ERK5 MAPK signaling transduction pathway and induce the expressions of apoptosis related proteins to inhibit the proliferation of MDA-MB-231 cells.
研究细胞外调节蛋白激酶5（ERK5）丝裂原活化蛋白激酶（MAPK）信号转导通路在染料木黄酮抑制人乳腺癌MDA-MB-231细胞中的作用。
采用MTT法观察染料木黄酮对MDA-MB-231细胞的抑制作用。通过流式细胞术检测细胞凋亡情况。采用蛋白质免疫印迹法检测ERK5 MAPK、Bax和Caspase3蛋白表达。
染料木黄酮抑制MDA-MB-231细胞增殖并诱导其凋亡。随着染料木黄酮剂量增加，ERK5蛋白表达降低，Bax和Caspase3蛋白表达增加。
染料木黄酮可影响ERK5 MAPK信号转导通路，诱导凋亡相关蛋白表达，从而抑制MDA-MB-231细胞增殖。
