[Development of ELISA-kit of quantitative analysis for Zearalenone].

OBJECTIVE

To develope a rapid, sensitive, quantitative ELISA-kit for Zearalenone and determine zearalenone in cereals.

METHODS

On the base of monoclonal antibodies against ZEN, apply indirect ELISA to study the performance parameter of the kit.

RESULTS

The limited concentration of detection of the ELISA-kit was 1ng/ml, linear range was 1-200 ng/ml, the linear equation was Y = 0.99 - 0.40 x (R2 = 0.99). The inhibition concentration of 50% against ZEN was 16.3 ng/ml. The average recovery rate of spiked corn and wheat was 96.5% and 95.5%, respectively, the coefficient of variant was 13.2% and 10.9%, respectively. The kit can be stored at 4 degrees C over 6 months. The cross reaction rate with the other mycotoxins was less than 1%, and coefficient of variant within-laboratory and between-laboratory was less than 15% and less than 20%, respectively. Detecting the VICAM sample with ELISA method and HPLC method, the results were within the range of the sample, and there was no statistic difference between the two methods.

CONCLUSION

This ELISA-kit was quick, sensitive, stable and specific and can be used to determine ZEN in cereals.