Choi Sung-Wook, Chang Hyun-Joo, Lee Nari, Kim Jae-Ho, Chun Hyang Sook
Korea Food Research Institute, San 516, Backhyun-dong, Bundang-gu, Sungnam, Kyonggi-do 463-746, Korea.
J Agric Food Chem. 2009 Feb 25;57(4):1113-8. doi: 10.1021/jf804022p.
The aim of the present work was to investigate the feasibility of employing the molecular imprinting polymer technique for detecting the mycotoxin zearalenone using a surface plasmon resonance (SPR) transducer. The molecularly imprinted polypyrrole (MIPPy) film was prepared by electropolymerization of pyrrole onto the bare Au chip in the presence of a template zearalenone molecule. The MIPPy-SPR sensor exhibited a linear response in the range of 0.3-3000 ng/mL (R (2) = 0.993) for detection of zearalenone. The selectivity efficiencies of zearalenone and other structurally related analogues were 1.0 and 0.15-0.27, respectively. The limit of detection and average recovery of blank corn matrix spiked with 30 ng/g zearalenone were 0.3 ng/g and 89%, respectively, and these were found to be comparable to those obtained by enzyme-linked immunosorbent assay. These results suggest that a combination of SPR sensing with MIPPy film is a promising alternative method for the detection of zearalenone.
本研究的目的是探讨采用分子印迹聚合物技术，利用表面等离子体共振（SPR）传感器检测霉菌毒素玉米赤霉烯酮的可行性。在模板玉米赤霉烯酮分子存在的情况下，通过吡咯在裸金芯片上的电聚合制备了分子印迹聚吡咯（MIPPy）膜。MIPPy-SPR传感器在检测玉米赤霉烯酮时，在0.3-3000 ng/mL范围内呈现线性响应（R (2) = 0.993）。玉米赤霉烯酮与其他结构相关类似物的选择性效率分别为1.0和0.15-0.27。在空白玉米基质中添加30 ng/g玉米赤霉烯酮时，检测限和平均回收率分别为0.3 ng/g和89%，发现这些结果与酶联免疫吸附测定法获得的结果相当。这些结果表明，SPR传感与MIPPy膜相结合是一种有前景的检测玉米赤霉烯酮的替代方法。
