肺炎球菌表面蛋白A（PspA）DNA和蛋白增强对肺炎球菌感染的保护性免疫
Enhanced protective immunity against pneumococcal infection with PspA DNA and protein.
作者信息
Moore Quincy C, Bosarge Joseph R, Quin Lisa R, McDaniel Larry S
机构信息
Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, United States.
出版信息
Vaccine. 2006 Jul 17;24(29-30):5755-61. doi: 10.1016/j.vaccine.2006.04.046. Epub 2006 May 4.
The effect of priming and boosting with pspA/EF5668 and purified recombinant PspA/EF5668 was examined. With this strategy CBA/N mice were protected against fatal challenge with Streptococcus pneumoniae EF5668. Anti-PspA antibody titers were elevated, and Western analysis with the immune serum demonstrated cross-reactivity with PspA from several different pneumococcal isolates, representing different PspA clades. Immune serum localized cross-reactive epitopes to the alpha-helical domain of PspA/Rx1 and PspA/EF5668. We demonstrated that DNA/protein prime-boost immunizations can enhance protective immunity against pneumococcal challenge.
研究了用pspA/EF5668和纯化的重组PspA/EF5668进行初免和加强免疫的效果。采用该策略，CBA/N小鼠受到了针对肺炎链球菌EF5668致死性攻击的保护。抗PspA抗体滴度升高，免疫血清的蛋白质免疫印迹分析表明与来自几种不同肺炎球菌分离株的PspA具有交叉反应性，这些分离株代表不同的PspA进化枝。免疫血清将交叉反应性表位定位到PspA/Rx1和PspA/EF5668的α-螺旋结构域。我们证明了DNA/蛋白质初免-加强免疫可增强针对肺炎球菌攻击的保护性免疫。
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