bcl-2家族成员在恶性胸膜间皮瘤中的表达。

Expression of bcl-2 family members in malignant pleural mesothelioma.

作者信息

O'Kane Sara L, Pound Rachelle J, Campbell Anne, Chaudhuri Nilanjan, Lind Michael J, Cawkwell Lynn

机构信息

Postgraduate Medical Institute of the University of Hull and Hull York Medical School, University of Hull, UK.

出版信息

Acta Oncol. 2006;45(4):449-53. doi: 10.1080/02841860500468927.

DOI:10.1080/02841860500468927

PMID:16760181

Abstract

Little is known about the Bcl-2 family members in mesothelioma. These proteins are involved in the control of apoptosis, carrying out both pro- and anti- apoptotic functions. Immunohistochemistry was used to examine the expression of p53 and Bcl-2 family members in 54 archival mesothelioma samples (39 epithelial, 15 sarcomatoid tumours). Overexpression of p53 was observed in 81% (44/54). For anti-apoptotic proteins, overexpression was recorded as follows: Bcl-2 40% (22/54), Bcl-XL 24% (13/54), Mcl-1 92% (50/54). For pro-apoptotic proteins, loss of expression was recorded as follows: Bad 25% (14/54), Bak 24% (13/54), Bax 42% (23/54), Bid 37% (20/54), Bim 18% (10/54). Statistically significant differences between epithelial and sarcomatoid tumours were observed for Bid (p < 0.001), Bad (p = 0.012) and Bcl-XL (p = 0.03). Significant differences in abnormal expression of apoptosis proteins were found between epithelial and sarcomatoid subtypes but histological subtype was the only factor with significant association to patient prognosis.

摘要

关于间皮瘤中的Bcl-2家族成员，人们了解甚少。这些蛋白质参与细胞凋亡的调控，兼具促凋亡和抗凋亡功能。采用免疫组织化学方法检测了54例存档间皮瘤样本（39例上皮型、15例肉瘤样肿瘤）中p53和Bcl-2家族成员的表达情况。81%（44/54）的样本中观察到p53过表达。对于抗凋亡蛋白，过表达情况如下：Bcl-2为40%（22/54），Bcl-XL为24%（13/54），Mcl-1为92%（50/54）。对于促凋亡蛋白，表达缺失情况如下：Bad为25%（14/54），Bak为24%（13/54），Bax为42%（23/54），Bid为37%（20/54），Bim为18%（10/54）。在上皮型和肉瘤样肿瘤之间，Bid（p < 0.001）、Bad（p = 0.012）和Bcl-XL（p = 0.03）存在统计学显著差异。在上皮型和肉瘤样亚型之间发现凋亡蛋白异常表达存在显著差异，但组织学亚型是与患者预后显著相关的唯一因素。