Boyd Amanda, Fazakerley John K, Bridgen Anne
Centre for Infectious Diseases, College of Medicine and Veterinary Medicine, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK.
Department of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, Northern Ireland, UK.
J Gen Virol. 2006 Jul;87(Pt 7):2005-2009. doi: 10.1099/vir.0.81767-0.
In 129 mice, infection with the nairovirus Dugbe virus (DUGV) was lethal following intracerebral but not intraperitoneal inoculation. Following both routes of inoculation, immunostaining of tissue sections demonstrated virus-positive cells in the brain, indicating that DUGV is neuroinvasive in mice. Many brain areas were affected and neurones were the main cell type infected. Infected cells showed punctate accumulations of viral nucleoprotein in the cytoplasm, indicative of virus replication sites. Immunostaining for activated caspase 3 demonstrated no evidence of apoptosis. The type I interferon (IFN) system plays a significant role in defence against DUGV, as 129 IFN-alpha/beta R(-/-) mice died rapidly following both intraperitoneal and intracerebral inoculations. Studies were undertaken to determine whether the IFN-inducible proteins, protein kinase R (PKR) and MxA, were important for protection; neither PKR nor constitutively expressed human MxA played significant roles.
在129只小鼠中，内罗病毒杜贝病毒（DUGV）经脑内接种而非腹腔接种具有致死性。经两种接种途径后，组织切片的免疫染色显示脑内有病毒阳性细胞，表明DUGV在小鼠中具有神经侵袭性。许多脑区受到影响，神经元是主要的被感染细胞类型。被感染细胞的细胞质中显示出病毒核蛋白的点状聚集，这表明是病毒复制位点。活化的半胱天冬酶3的免疫染色未显示凋亡迹象。I型干扰素（IFN）系统在抵御DUGV中发挥重要作用，因为129只IFN-α/β R（-/-）小鼠在腹腔和脑内接种后均迅速死亡。开展了研究以确定IFN诱导蛋白、蛋白激酶R（PKR）和MxA是否对保护作用重要；PKR和组成型表达的人MxA均未发挥显著作用。
