Rindorf Lars, Høiby Poul Erik, Jensen Jesper Bo, Pedersen Lars Hagsholm, Bang Ole, Geschke Oliver
COM.DTU, Department of Communication, Optics and Materials, Technical University of Denmark, 2800, Kongens Lyngby, Denmark.
Anal Bioanal Chem. 2006 Aug;385(8):1370-5. doi: 10.1007/s00216-006-0480-8. Epub 2006 Jun 8.
In this paper we present the first incorporation of a microstructured optical fiber (MOF) into biochip applications. A 16-mm-long piece of MOF is incorporated into an optic-fluidic coupler chip, which is fabricated in PMMA polymer using a CO(2) laser. The developed chip configuration allows the continuous control of liquid flow through the MOF and simultaneous optical characterization. While integrated in the chip, the MOF is functionalized towards the capture of a specific single-stranded DNA string by immobilizing a sensing layer on the microstructured internal surfaces of the fiber. The sensing layer contains the DNA string complementary to the target DNA sequence and thus operates through the highly selective DNA hybridization process. Optical detection of the captured DNA was carried out using the evanescent-wave-sensing principle. Owing to the small size of the chip, the presented technique allows for analysis of sample volumes down to 300 nL and the fabrication of miniaturized portable devices.
在本文中，我们首次将微结构光纤（MOF）应用于生物芯片。将一段16毫米长的MOF集成到一个光流体耦合器芯片中，该芯片采用CO(2)激光在聚甲基丙烯酸甲酯（PMMA）聚合物中制造。所开发的芯片配置允许对通过MOF的液体流动进行连续控制并同时进行光学表征。集成在芯片中的MOF通过在光纤微结构内表面固定传感层，实现了对特定单链DNA序列的捕获功能化。传感层包含与目标DNA序列互补的DNA链，因此通过高度选择性的DNA杂交过程发挥作用。利用倏逝波传感原理对捕获的DNA进行光学检测。由于芯片尺寸小，所提出的技术能够分析低至300纳升的样品体积，并制造小型便携式设备。
