Holder Geoff
Department of Clinical Biochemistry, Regional Endocrine Laboratory, University Hospital Birmingham NHS Trust, UK.
Methods Mol Biol. 2006;324:141-57. doi: 10.1385/1-59259-986-9:141.
The background to the physiological production of glucocorticoids is described as well as their concentrations in some biological fluids. Detailed methods are described for the collection, storage, and preparation of samples and for the immunoassay techniques for some common steroids in serum, urine, and saliva. Alternative methods (high-performance liquid chromatography, gas-liquid chromatography, Gas chromatography-mass spectrometry) that have greater specificity and/or sensitivity are also discussed, together with automated procedures able to analyse large numbers of samples rapidly. Information and references are presented on the analysis of less commonly measured glucocorticoids, including synthetic steroids such as dexamethasone and prednisolone.
本文描述了糖皮质激素生理产生的背景及其在一些生物体液中的浓度。详细介绍了样本的采集、储存和制备方法，以及血清、尿液和唾液中一些常见类固醇的免疫分析技术。还讨论了具有更高特异性和/或灵敏度的替代方法（高效液相色谱法、气液色谱法、气相色谱 - 质谱法），以及能够快速分析大量样本的自动化程序。文中还提供了关于较少测定的糖皮质激素分析的信息和参考文献，包括地塞米松和泼尼松龙等合成类固醇。
