Robards K, Towers P
School of Science and Technology, Charles Sturt University-Riverina, Wagga Wagga, Australia.
Biomed Chromatogr. 1990 Jan;4(1):1-19. doi: 10.1002/bmc.1130040102.
Chromatographic methods (paper chromatography, thin layer chromatography, high performance liquid chromatography, gas chromatography and gas chromatography-mass spectrometry) for the determination of clinically important steroids in biological specimens are reviewed. The emphasis is on the use of gas chromatography, gas chromatography-mass spectrometry and high performance liquid chromatography as reference rather than routine techniques. Chromatographic methods are compared with colorimetric, fluorimetric and radioimmunoassay procedures in terms of simplicity of operation, cost and ability to analyse large numbers of specimens. The importance of correct specimen collection and storage are discussed. Sample preparation techniques for the various analytical methods are described. These include extraction of free and conjugated steroids from serum, plasma, urine and saliva by solvent partition, with polymer-based resins such as Amberlite XAD-2, DEAE-Sephadex and Sephadex resins bonded with various other function groups and, more recently, with chemically bonded reversed-phase silicas.
本文综述了用于测定生物样本中具有临床重要意义的甾体类化合物的色谱方法（纸色谱法、薄层色谱法、高效液相色谱法、气相色谱法以及气相色谱 - 质谱联用技术）。重点在于气相色谱法、气相色谱 - 质谱联用技术和高效液相色谱法作为参考方法而非常规技术的应用。就操作简便性、成本以及分析大量样本的能力而言，将色谱方法与比色法、荧光法和放射免疫分析法进行了比较。讨论了正确采集和储存样本的重要性。描述了各种分析方法的样本制备技术。这些技术包括通过溶剂分配从血清、血浆、尿液和唾液中提取游离和结合型甾体类化合物，使用基于聚合物的树脂，如Amberlite XAD - 2、DEAE - 葡聚糖以及与各种其他官能团结合的葡聚糖树脂，以及最近使用的化学键合反相硅胶。
